Rabbit anti-Human POLR2B Polyclonal Antibody | anti-POLR2B antibody

Anti-POLR2B Antibody, Rabbit Polyclonal

Cat. Num. AAA8109553

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: POLR2B; Polyclonal Antibody; Anti-POLR2B Antibody; Rabbit Polyclonal; POLR2B Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-hRPB140 Antibody; Anti-POL2RB Antibody; Anti-RPB2 Antibody; anti-POLR2B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human POLR2B
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-POLR2B antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human POLR2B fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human POLR2B fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-POLR2B rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:134 kDa Observed band size:130 kDa)

Immunoprecipitation (IP)

(POLR2B was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-POLR2B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-POLR2B rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 130 kDa Observed band size :130 kDa)

Related Product Information for anti-POLR2B antibody

POLR2B (RNA Polymerase II Subunit B, also named RPB2) is a Protein Coding gene. The POLR2B gene, located on 4q12, is conserved in chimpanzee, Rhesus monkey, dog, and cow, etc. This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA, and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Diseases associated with POLR2B include Fusariosis and Eumycotic Mycetoma. Among its related pathways are RNA Polymerase II Transcription Initiation And Promoter Clearance and Transcription-Coupled Nucleotide Excision Repair (TC-NER).

References

Palangat M, et al. (2011) The rpb2 flap loop of human rna polymerase ii is dispensable for transcription initiation and elongation. Mol Cell Biol 31 (16): 3312-3325.Luo J, et al. (2007) Duplication and paralog sorting of rpb2 and rpb1 genes in core eudicots. Mol Phylogenet Evol 44 (2): 850-862.Langelier MF, et al. (2005) The highly conserved glutamic acid 791 of rpb2 is involved in the binding of ntp and mg(b) in the active center of human rna polymerase ii. Nucleic Acids Res 33 (8): 2629-2639.