Western Blot (WB)
(Anti-ICMT rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:32 kDaObserved band size:32 kDa)
Rabbit ICMT Polyclonal Antibody | anti-ICMT antibody
Anti-ICMT Antibody, Rabbit Polyclonal
Reactivity
Human, Cynomolgus
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
ICMT; Polyclonal Antibody; Anti-ICMT Antibody; Rabbit Polyclonal; ICMT Antibody; Rabbit PAb; Antigen Affinity Purified; isoprenylcysteine carboxyl methyltransferase; Anti-HSTE14 Antibody; Anti-MST098 Antibody; Anti-MSTP098 Antibody; Anti-PCCMT Antibody; Anti-PCMT Antibody; Anti-PPMT Antibody; anti-ICMT antibody
Host
Rabbit
Reactivity
Human, Cynomolgus
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ICMT
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-ICMT antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:100-1:400
IHC-P: 1:100-1:400
Immunogen
E Coli-derived Human ICMT fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human ICMT fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-ICMT rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:32 kDaObserved band size:32 kDa)
Western Blot (WB)
(Anti-ICMT rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:32 kDaObserved band size:32 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining ICMT in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining ICMT in human kidney with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining ICMT in cynomolgus cerebellum with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining ICMT in cynomolgus cerebellum with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-ICMT antibody
Isoprenylcysteine carboxyl methyltransferase (ICMT), a eukaryote-specific integral membrane enzyme that resides in the endoplasmic reticulum. ICMT is the only cellular enzyme that is known to methylate prenylcysteine substrates; methylation is important for the biological functions of these substrates, such as the membrane localization and subsequent activity of RAS, prelamin A and RAB. Inhibition of ICMT has a potential for combating progeria and cancer. ICMT plays a key role in post-translational regulation of prenylated proteins. ICMT methylates the carboxylate of a lipid-modified cysteine at the C terminus of its protein substrates. This is the final post-translational modification of proteins containing a CAAX motif, including the oncoprotein Ras, and therefore, ICMT may serve as a therapeutic target in cancer development.
References
Diver MM, et al. (2018) Atomic structure of the eukaryotic intramembrane ras methyltransferase icmt. Nature 553 (7689): 526-529.
Diver MM, et al. (2014) Mutational analysis of the integral membrane methyltransferase isoprenylcysteine carboxyl methyltransferase (icmt) reveals potential substrate binding sites. J Biol Chem 289 (38): 26007-26020.
Bergo MO, et al. (2004) Inactivation of icmt inhibits transformation by oncogenic k-ras and b-raf. J Clin Invest 113 (4): 539-550.
Diver MM, et al. (2014) Mutational analysis of the integral membrane methyltransferase isoprenylcysteine carboxyl methyltransferase (icmt) reveals potential substrate binding sites. J Biol Chem 289 (38): 26007-26020.
Bergo MO, et al. (2004) Inactivation of icmt inhibits transformation by oncogenic k-ras and b-raf. J Clin Invest 113 (4): 539-550.
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Product Notes
The ICMT (Catalog #AAA8108680) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ICMT Antibody, Rabbit Polyclonal reacts with Human, Cynomolgus and may cross-react with other species as described in the data sheet. AAA Biotech's ICMT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:100-1:400. Researchers should empirically determine the suitability of the ICMT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ICMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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