Rabbit POLA1 Polyclonal Antibody | anti-POLA1 antibody

POLA1 Antibody (C-Term)

Cat. Num. AAA9231251

• Gene Names: POLA1; NSX; POLA; p180
• Reactivity: Mouse; Predicted: Human
• Applications: Western Blot, ELISA
• Purity: Purified through a protein A column, followed by peptide affinity purification.
• Synonyms: POLA1; Polyclonal Antibody; POLA1 Antibody (C-Term); Purified Rabbit Polyclonal Antibody (Pab); DNA polymerase alpha catalytic subunit; 2.7.7.7; DNA polymerase alpha catalytic subunit p180; POLA; anti-POLA1 antibody

• Host: Rabbit
• Reactivity: Mouse; Predicted: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This POLA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1406-1439 amino acids from the human region of human POLA1.
• Purity/Purification: Purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 1462

• Applicable Applications for anti-POLA1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:2000
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Cellular Location: Nucleus. Cytoplasm, cytosol. Note=In the cytosol, colocalizes with RNA:DNA hybrids with a speckled pattern
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks.; For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-POLA1 Antibody (C-Term) at 1:2000 dilution + NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 166 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-POLA1 antibody

Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses (PubMed:27019227).

Product Categories/Family for anti-POLA1 antibody

Primary Antibodies

References

Wong S.W.,et al.EMBO J. 7:37-47(1988). Pearson B.E.,et al.Mol. Cell. Biol. 11:2081-2095(1991). Hsi K.-L.,et al.Nucleic Acids Res. 18:6231-6237(1990). Smale S.T.,et al.Mol. Cell. Biol. 6:4077-4087(1986). Lee S.S.,et al.Proc. Natl. Acad. Sci. U.S.A. 92:7882-7886(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 106507301
• NCBI GeneID: 5422
• NCBI Accession #: NP_058633.2
• NCBI GenBank Nucleotide #: NM_016937.3
• UniProt Accession #: P09884; Q86UQ7
• Molecular Weight: 165913
• NCBI Official Full Name: DNA polymerase alpha catalytic subunit isoform 2
• NCBI Official Synonym Full Names: DNA polymerase alpha 1, catalytic subunit
• NCBI Official Symbol: POLA1
• NCBI Official Synonym Symbols: NSX; POLA; p180
• NCBI Protein Information: DNA polymerase alpha catalytic subunit
• UniProt Protein Name: DNA polymerase alpha catalytic subunit
• Protein Family: DNA polymerase
• UniProt Gene Name: POLA1
• UniProt Synonym Gene Names: POLA

NCBI Description

This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010]

Uniprot Description

Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses (PubMed:27019227).