Rabbit anti-Human DNA Polymerase alpha Polyclonal Antibody | anti-POLA1 antibody

DNA Polymerase alpha Antibody

Cat. Num. AAA9604545

• Gene Names: POLA1; NSX; POLA; p180
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: DNA Polymerase alpha; Polyclonal Antibody; DNA Polymerase alpha Antibody; DKFZp686K1672; DNA polymerase alpha 1 catalytic subunit; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; DNA polymerase alpha p180 subunit; DPOLA_HUMAN; OTTHUMP00000023089; OTTHUMP00000023090; P180; p180 subunit; POLA; POLA1; Polymerase (DNA directed); alpha 1; catalytic subunit; Polymerase (DNA-directed); alpha (70kD); anti-POLA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DNA Polymerase alpha antibody detects endogenous levels of total DNA Polymerase alpha
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1462

• Applicable Applications for anti-POLA1 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide
• Subcellular Location: Nucleus.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunofluorescence (IF)

(MBS9604545 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Western Blot (WB)

(Western blot analysis of extracts from Jurkat cells using DNA Polymerase alpha antibody.)

Related Product Information for anti-POLA1 antibody

Function: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses (PubMed:27019227).
Subunit Structure: The DNA polymerase alpha complex is composed of four subunits: the catalytic subunit POLA1, the regulatory subunit POLA2, and the small and the large primase subunits PRIM1 and PRIM2 respectively. Interacts with PARP1; this interaction functions as part of the control of replication fork progression. Interacts with MCM10 and WDHD1; these interactions recruit the polymerase alpha complex to the pre-replicative complex bound to DNA. Interacts with RPA1; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interacts with SV40 Large T antigen; this interaction allows viral DNA replication. Interacts with herpes simplex virus 1 (HHV-1) replication origin-binding protein UL9.
Post-translational Modifications: A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.
Similarity: The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes. Belongs to the DNA polymerase type-B family.

NCBI and Uniprot Product Information

• NCBI GI #: 106507301
• NCBI GeneID: 5422
• NCBI Accession #: NP_058633.2
• NCBI GenBank Nucleotide #: NM_016937.3
• UniProt Accession #: P09884; Q86UQ7
• Molecular Weight: Observed: 165 kD; Predicted: 166 kDa
• NCBI Official Full Name: DNA polymerase alpha catalytic subunit isoform 2
• NCBI Official Synonym Full Names: DNA polymerase alpha 1, catalytic subunit
• NCBI Official Symbol: POLA1
• NCBI Official Synonym Symbols: NSX; POLA; p180
• NCBI Protein Information: DNA polymerase alpha catalytic subunit
• UniProt Protein Name: DNA polymerase alpha catalytic subunit
• Protein Family: DNA polymerase
• UniProt Gene Name: POLA1
• UniProt Synonym Gene Names: POLA

NCBI Description

This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010]

Uniprot Description

Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses (PubMed:27019227).