Rabbit TRIP10 Polyclonal Antibody | anti-TRIP10 antibody

TRIP10 Antibody

Cat. Num. AAA9608549

• Gene Names: TRIP10; STP; CIP4; HSTP; STOT; TRIP-10
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: TRIP10; Polyclonal Antibody; TRIP10 Antibody; Cdc42 interacting protein 4; Cdc42 interaction protein 4 long isoform; Cdc42-interacting protein 4; CG11341; CG15015 PA; Cip 4; CIP4; CIP4_HUMAN; DCIP4; hSTP; Protein Felic; Salt tolerant protein; Salt tolerator; STOT; STP; Thyroid hormone receptor interactor 10; Thyroid receptor interacting protein 10; Thyroid receptor-interacting protein 10; TR-interacting protein 10; TRIP 10; TRIP-10; trip10; Truncated Cdc42 interaction protein 4; anti-TRIP10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TRIP10 antibody detects endogenous levels of total TRIP10
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 601

• Applicable Applications for anti-TRIP10 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human TRIP10
• Subcellular Location: Cytoplasm > Perinuclear Region and Cytoplasm > Cytoskeleton. Cytoplasm > Cell Cortex. Lysosome. Golgi Apparatus. Cell Membrane. Cell projection > Phagocytic Cup. Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ (By similarity). Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9.
• Tissue Specificity: Expressed in brain, colon, heart, kidney, liver, lung, megakaryocyte, ovary, pancreas, peripheral blood lymphocytes, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus and trachea.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Dog
• Similarity: Pig (92%), Bovine (92%), Horse (92%), Dog (92%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of TRIP10 using HuvEc whole cell lysates)

Related Product Information for anti-TRIP10 antibody

Function: Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4, 5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.
Subunit Structure: Interacts specifically with GTP-bound RHOQ. Interacts with DNM2 and PDE6G (By similarity). Homodimerizes, the dimers can polymerize end-to-end to form filamentous structures. Interacts specifically with GTP-bound CDC42. Interacts with AKAP9, ARHGAP17, DAAM1, DIAPH1, DIAPH2, DNM1, FASLG/FASL, GAPVD1, LYN, microtubules, SRC, WAS/WASP and WASL/N-WASP. Interacts with the ligand binding domain of the thyroid receptor (TR) in the presence of thyroid hormone. May interact with CTNNB1 and HD/HTT.
Post-translational Modifications: Tyrosine phosphorylated. Also phosphorylated by PKA.
Similarity: The F-BAR domain binds the phospholipid membrane with its concave surface. The end-to-end polymerization of dimers of these domains provides a curved surface that fits best membranes with around 600 A diameter, and may drive tubulation. Belongs to the FNBP1 family.

NCBI and Uniprot Product Information

• NCBI GI #: 572874682
• NCBI GeneID: 9322
• NCBI Accession #: NP_001275891.1
• NCBI GenBank Nucleotide #: NM_001288962.1
• UniProt Accession #: Q15642; O15184; Q53G22; Q5TZN1; Q6FI24; Q8NFL1; Q8TCY1; Q8TDX3; Q96RJ1; B2R8A6; B7WP22; D6W645
• Molecular Weight: Observed: 68 kDa; Predicted: 69 kDa
• NCBI Official Full Name: cdc42-interacting protein 4 isoform 1
• NCBI Official Synonym Full Names: thyroid hormone receptor interactor 10
• NCBI Official Symbol: TRIP10
• NCBI Official Synonym Symbols: STP; CIP4; HSTP; STOT; TRIP-10
• NCBI Protein Information: cdc42-interacting protein 4
• UniProt Protein Name: Cdc42-interacting protein 4
• Protein Family: Cdc42-interacting protein
• UniProt Gene Name: TRIP10
• UniProt Synonym Gene Names: CIP4; STOT; STP; hSTP; TR-interacting protein 10; TRIP-10

Uniprot Description

Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.