Western Blot (WB)
(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane: 293T cell Primary antibody: 1/250 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)
Rabbit POLA1 Polyclonal Antibody | anti-POLA1 antibody
POLA1 Antibody
Western Blot (WB)
(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane: 293T cell Primary antibody: 1/250 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)
Western Blot (WB)
(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane: 293T cell Primary antibody: 1/250 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds)
NCBI and Uniprot Product Information
Uniprot Description
POLA: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. Belongs to the DNA polymerase type-B family.
Protein type: DNA repair, damage; DNA replication; EC 2.7.7.7; Nuclear envelope; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transferase
Chromosomal Location of Human Ortholog: Xp22.1-p21.3
Cellular Component: alpha DNA polymerase:primase complex; chromatin; cytoplasm; nuclear envelope; nuclear matrix; nucleolus; nucleoplasm; nucleus
Molecular Function: chromatin binding; DNA binding; DNA primase activity; DNA-directed DNA polymerase activity; nucleotide binding; protein binding; protein kinase binding
Biological Process: cell proliferation; DNA repair; DNA replication; DNA replication initiation; DNA strand elongation during DNA replication; DNA synthesis during DNA repair; double-strand break repair via nonhomologous end joining; G1/S transition of mitotic cell cycle; G1/S-specific transcription in mitotic cell cycle; lagging strand elongation; leading strand elongation; telomere maintenance via recombination
Disease: N Syndrome; Pigmentary Disorder, Reticulate, With Systemic Manifestations, X-linked
Similar Products
Product Notes
The POLA1 pola1 (Catalog #AAA9416480) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POLA1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's POLA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 1:200-1:1000. Researchers should empirically determine the suitability of the POLA1 pola1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
