Rabbit PLEKHM3 Polyclonal Antibody | anti-PLEKHM3 antibody

PLEKHM3 Antibody

Cat. Num. AAA151324

• Gene Names: PLEKHM3; DAPR; PLEKHM1L
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: PLEKHM3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: PLEKHM3; Polyclonal Antibody; PLEKHM3 Antibody; DAPR; PLEKHM1L; Pleckstrin homology domain-containing family M member 3; PH domain-containing family M member 3; pleckstrin homology domain containing; family M; member 3; anti-PLEKHM3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: PLEKHM3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 761

• Applicable Applications for anti-PLEKHM3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: PLEKHM3 antibody can be used for detection of PLEKHM3 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: PLEKHM3 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human PLEKHM3.
• Buffer: PLEKHM3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: PLEKHM3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PLEKHM3 in mouse skeletal muscle tissue lysate with PLEKHM3 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of PLEKHM3 in human brain tissue with PLEKHM3 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of PLKHM3 in human brain tissue with PLKHM3 antibody at 20 μg/mL.)

Related Product Information for anti-PLEKHM3 antibody

PLEKHM3 Antibody: PLEKHM3, also known as DAPR, is a member of the M family of Pleckstrin homology domain-containing proteins. PLEKHM3 was initially identified through chromatin immunoprecipitation and CpG microarray analysis examining proteins regulated by myocyte-enhancing factor 2. In C2C12 myoblast cells, PLEKHM3 binds to the PI3K signaling member protein kinase B in the cytosol prior to differentiation into myotubes. Following the initiation of differentiation, PLEKHM3 was also found in membrane fractions. Knockdown of PLEKHM3 expression by RNAi resulted in the inhibition of myotube formation, suggesting that PLEKHM3 is a key component required by myoblasts for orchestrating their differentiation during myogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 172046173
• NCBI GeneID: 389072
• UniProt Accession #: Q6ZWE6; Q8WW68; B9EKV2
• Molecular Weight: 59,587 Da
• NCBI Official Full Name: Pleckstrin homology domain-containing family M member 3
• NCBI Official Synonym Full Names: pleckstrin homology domain containing, family M, member 3
• NCBI Official Symbol: PLEKHM3
• NCBI Official Synonym Symbols: DAPR; PLEKHM1L
• NCBI Protein Information: pleckstrin homology domain-containing family M member 3; differentiation associated protein; PH domain-containing family M member 3
• UniProt Protein Name: Pleckstrin homology domain-containing family M member 3
• Protein Family: Pleckstrin homology domain-containing family
• UniProt Gene Name: PLEKHM3
• UniProt Synonym Gene Names: PLEKHM1L; PH domain-containing family M member 3
• UniProt Entry Name: PKHM3_HUMAN

Uniprot Description

PLEKHM3: 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 2q33.3

Molecular Function: metal ion binding