Rabbit anti-Human, Rat TIAL1 Polyclonal Antibody | anti-TIAL1 antibody

TIAL1 (TIA1 Cytotoxic Granule-associated RNA Binding Protein-like 1, T-cluster Binding Protein, TCBP, TIAR) (HRP)

Cat. Num. AAA6440657

• Gene Names: TIAL1; TCBP; TIAR
• Reactivity: Human, Rat
• Applications: Immunoprecipitation, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: TIAL1; Polyclonal Antibody; TIAL1 (TIA1 Cytotoxic Granule-associated RNA Binding Protein-like 1; T-cluster Binding Protein; TCBP; TIAR) (HRP); anti-TIAL1 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes TIAL1. Species Crossreactivity: human, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-TIAL1 antibody: Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 1:20-1:50; WB: 1:500-1:1000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human TIAL1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of K562 cell line, using TIAL1 antibody.)

Product Categories/Family for anti-TIAL1 antibody

Antibodies; RNA Processing

NCBI and Uniprot Product Information

• NCBI GI #: 267131
• NCBI GeneID: 7073
• UniProt Accession #: Q01085; A8K3T0; A8K4L9
• Molecular Weight: 375
• NCBI Official Full Name: Nucleolysin TIAR
• NCBI Official Synonym Full Names: TIA1 cytotoxic granule-associated RNA binding protein-like 1
• NCBI Official Symbol: TIAL1
• NCBI Official Synonym Symbols: TCBP; TIAR
• NCBI Protein Information: nucleolysin TIAR; T-cluster binding protein; TIA-1-related nucleolysin; aging-associated gene 7 protein
• UniProt Protein Name: Nucleolysin TIAR
• Protein Family: Nucleolysin
• UniProt Gene Name: TIAL1
• UniProt Entry Name: TIAR_HUMAN

NCBI Description

The protein encoded by this gene is a member of a family of RNA-binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing. [provided by RefSeq, Jul 2008]

Uniprot Description

TIAL1: a member of a family of RNA-binding proteins that possesses nucleolytic activity. A cytotoxic T cell granule-associated protein that has been shown to bind specifically to poly(A) homopolymers and to fragment DNA in permeabilized target cells. Members of this protein family may be involved in the induction of apoptosis. Two splice variant isoforms have been described.

Protein type: RNA-binding; Apoptosis

Chromosomal Location of Human Ortholog: 10q

Cellular Component: stress granule; lysosome; cytoplasm; nucleus

Molecular Function: RNA binding; nucleotide binding; AU-rich element binding

Biological Process: regulation of transcription from RNA polymerase II promoter; apoptosis; stem cell division; positive regulation of cell proliferation; defense response; germ cell development