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Western Blot (WB) (Western blot analysis of CDX2 in mouse brain tissue lysate with CDX2 antibody at 1 μg/mL.)

Rabbit CDX2 Polyclonal Antibody | anti-CDX2 antibody

CDX2 Antibody

Gene Names
CDX2; CDX3; CDX-3; CDX2/AS
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
CDX2 Antibody is affinity chromatography purified via peptide column.
Synonyms
CDX2; Polyclonal Antibody; CDX2 Antibody; CDX3; CDX-3; Homeobox protein CDX-2; caudal type homeobox 2; anti-CDX2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
CDX2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
313
Applicable Applications for anti-CDX2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
CDX2 antibody can be used for detection of CDX2 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
CDX2 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human CDX2.
Buffer
CDX2 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
CDX2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of CDX2 in mouse brain tissue lysate with CDX2 antibody at 1 μg/mL.)

Western Blot (WB) (Western blot analysis of CDX2 in mouse brain tissue lysate with CDX2 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of CDX2 in rat brain tissue with CDX2 antibody at 5 μg/mL.)

Immunohistochemistry (IHC) (Immunohistochemistry of CDX2 in rat brain tissue with CDX2 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of CDX2 in rat brain tissue with CDX2 antibody at 20 μg/mL.)

Immunofluorescence (IF) (Immunofluorescence of CDX2 in rat brain tissue with CDX2 antibody at 20 μg/mL.)
Related Product Information for anti-CDX2 antibody
CDX2 Antibody: CDX2 is a member of the caudal homeobox family of transcription factors that also includes the proteins CDX1 and CDX4. CDX2 is strongly expressed in the trophectoderm in the blastocyst with expression maintained within the proliferating extra-embryonic ectoderm during development. It is required for the repression of the transcription of the POU5F1/Oct4 and NANOG genes in the outer cells of the blastocyst and is thus essential for the segregation of the inner cell mass and trophectoderm at the blastocyst stage. Aberrant expression of CDX2 is associated with numerous types of carcinomas, including intestinal metaplasia, gastric carcinoma, and acute myeloid leukemia due to deregulated HOX gene expression.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
33,520 Da
NCBI Official Full Name
caudal-type homeobox protein 2
NCBI Official Synonym Full Names
caudal type homeobox 2
NCBI Official Symbol
CDX2
NCBI Official Synonym Symbols
CDX3; CDX-3; CDX2/AS
NCBI Protein Information
homeobox protein CDX-2; caudal-type homeobox protein 2; caudal type homeobox transcription factor 2; caudal type homeo box transcription factor 2
UniProt Protein Name
Homeobox protein CDX-2
Protein Family
UniProt Gene Name
CDX2
UniProt Synonym Gene Names
CDX3
UniProt Entry Name
CDX2_HUMAN

NCBI Description

This gene is a member of the caudal-related homeobox transcription factor gene family. The encoded protein is a major regulator of intestine-specific genes involved in cell growth an differentiation. This protein also plays a role in early embryonic development of the intestinal tract. Aberrant expression of this gene is associated with intestinal inflammation and tumorigenesis. [provided by RefSeq, Jan 2012]

Uniprot Description

CDX2: Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Belongs to the Caudal homeobox family.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: nucleoplasm; condensed nuclear chromosome; transcriptional repressor complex

Molecular Function: double-stranded DNA binding; transcription factor activity; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; establishment and/or maintenance of epithelial cell polarity; anterior/posterior pattern formation; blood vessel development; organ morphogenesis; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; endosome to lysosome transport; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; trophectodermal cell differentiation

Research Articles on CDX2

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Product Notes

The CDX2 cdx2 (Catalog #AAA150051) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDX2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CDX2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). CDX2 antibody can be used for detection of CDX2 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the CDX2 cdx2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDX2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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