Mouse Insulin Monoclonal Antibody | anti-INS antibody

Insulin, Proinsulin (APC)

Cat. Num. AAA6268905

• Gene Names: INS; ILPR; IRDN; IDDM2; MODY10
• Applications: Immunohistochemistry
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Insulin; Monoclonal Antibody; Proinsulin (APC); ILPR; Insulin Precursor; INS; IRDN; Proinsulin Precursor; anti-INS antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10B2426
• Specificity: Recognizes both human insulin and proinsulin, but does not react with free C-peptide. Species crossreactivity: mouse, rat, bovine, porcine
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-INS antibody: FLISA, Immunohistochemistry (IHC)
• Immunogen: Recombinant protein corresponding to human Insulin.
• Conjugate: APC
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry staining of paraffin embedded human pancreas using I7660-30L.)

Product Categories/Family for anti-INS antibody

Antibodies; Insulin

NCBI and Uniprot Product Information

• NCBI GI #: 386828
• NCBI GeneID: 3630
• UniProt Accession #: P01308; Q5EEX2
• Molecular Weight: 11,981 Da
• NCBI Official Full Name: insulin
• NCBI Official Synonym Full Names: insulin
• NCBI Official Symbol: INS
• NCBI Official Synonym Symbols: ILPR; IRDN; IDDM2; MODY10
• NCBI Protein Information: insulin; proinsulin; preproinsulin
• UniProt Protein Name: Insulin
• Protein Family: Insulin
• UniProt Gene Name: INS
• UniProt Entry Name: INS_HUMAN

NCBI Description

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Uniprot Description

Insulin: Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds. Belongs to the insulin family.

Protein type: Secreted, signal peptide; Hormone; Secreted

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: extracellular space; Golgi lumen; endoplasmic reticulum lumen; extracellular region

Molecular Function: insulin-like growth factor receptor binding; identical protein binding; protein binding; protease binding; hormone activity; insulin receptor binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of vasodilation; glucose homeostasis; activation of NF-kappaB transcription factor; positive regulation of glucose import; negative regulation of protein oligomerization; regulation of protein localization; cell-cell signaling; negative regulation of gluconeogenesis; acute-phase response; regulation of transmembrane transporter activity; negative regulation of NAD(P)H oxidase activity; positive regulation of mitosis; positive regulation of nitric-oxide synthase activity; activation of protein kinase B; negative regulation of acute inflammatory response; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of protein catabolic process; regulation of amino acid metabolic process; positive regulation of cell differentiation; negative regulation of glycogen catabolic process; wound healing; positive regulation of cellular protein metabolic process; positive regulation of glycogen biosynthetic process; glucose transport; regulation of protein secretion; regulation of transcription, DNA-dependent; negative regulation of lipid catabolic process; negative regulation of vasodilation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; negative regulation of proteolysis; negative regulation of protein secretion; MAPKKK cascade; negative regulation of fatty acid metabolic process; alpha-beta T cell activation; endocrine pancreas development; positive regulation of protein kinase B signaling cascade; G-protein coupled receptor protein signaling pathway; cellular protein metabolic process; fatty acid homeostasis; positive regulation of glycolysis; insulin receptor signaling pathway; energy reserve metabolic process; positive regulation of insulin receptor signaling pathway; regulation of insulin secretion; positive regulation of DNA replication; positive regulation of cytokine secretion; positive regulation of cell migration

Disease: Diabetes Mellitus, Insulin-dependent, 2; Diabetes Mellitus, Permanent Neonatal; Hyperproinsulinemia; Maturity-onset Diabetes Of The Young, Type 10