Rabbit ZNF536 Polyclonal Antibody | anti-ZNF536 antibody

ZNF536 Antibody

Cat. Num. AAA150992

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: ZNF536 Antibody is affinity chromatography purified via peptide column.
• Synonyms: ZNF536; Polyclonal Antibody; ZNF536 Antibody; KIAA0390; Zinc finger protein 536; zinc finger protein 536; anti-ZNF536 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: ZNF536 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1300

• Applicable Applications for anti-ZNF536 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: ZNF536 antibody can be used for detection of ZNF536 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: ZNF536 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ZNF536.
• Buffer: ZNF536 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: ZNF536 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ZNF536 in human brain tissue lysate with ZNF536 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ZNF536 in human brain tissue with ZNF536 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of ZNF536 in human brain tissue with ZNF536 antibody at 20 μg/mL.)

Related Product Information for anti-ZNF536 antibody

ZNF536 Antibody: ZNF536 is a recently identified zinc-finger protein that is expressed primarily in the developing nervous system and the cerebral cortex, hippocampus, and hypothalamus. ZNF536 possess ten zinc fingers and interacts with CtBP1, a corepressor for gene transcription. It is most closely related to transcriptional repressor ZNF219. Overexpression of ZNF536 in embryonic stem cells dramatically reduced the mRNA levels of neuronal marker genes such as Pax6, MAP2, and beta-tubulin III following retinoic acid (RA)-induced differentiation, while depletion of ZNF536 via RNAi resulted in elevated mRNA levels of these genes, indicating its role in inhibiting neuronal cell differentiation. Overexpression of RA receptor a rescues the inhibitory role of ZNF536, suggesting that ZNF536 might inhibit RA response element-mediated transcriptional activity.

NCBI and Uniprot Product Information

• NCBI GI #: 7662092
• NCBI GeneID: 9745
• NCBI Accession #: NP_055532.1
• NCBI GenBank Nucleotide #: NM_014717.1
• UniProt Accession #: O15090; A2RU18
• Molecular Weight: 141,417 Da
• NCBI Official Full Name: zinc finger protein 536
• NCBI Official Synonym Full Names: zinc finger protein 536
• NCBI Official Symbol: ZNF536
• NCBI Protein Information: zinc finger protein 536
• UniProt Protein Name: Zinc finger protein 536
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZNF536
• UniProt Synonym Gene Names: KIAA0390
• UniProt Entry Name: ZN536_HUMAN

Uniprot Description

ZNF536: May be involved in transcriptional regulation. Recognizes and binds 2 copies of the core DNA sequence 5'-CCCCCA- 3'. Belongs to the krueppel C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 19q12

Cellular Component: nucleus

Molecular Function: metal ion binding

Biological Process: negative regulation of retinoic acid receptor signaling pathway; negative regulation of neuron differentiation; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter