Western Blot (WB)
(Sample Data: Western blot using Anti-Phospho-APC6 (Thr580) antibody (1:1,000) shows detection of a band ~72 kDa corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate. Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35ug of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose.)
Rabbit anti-Human Phospo-APC6 Polyclonal Antibody | anti-Phospo-APC6 antibody
Anti-Phospo-APC6 (Thr580)
Reactivity
Human
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Affinity chromatography
Synonyms
Phospo-APC6; Polyclonal Antibody; Anti-Phospo-APC6 (Thr580); anti-Phospo-APC6 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the APC6 protein phosphorylated at Threonine 580
Purity/Purification
Affinity chromatography
Form/Format
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH7.2 + 0.01% (w/v) Sodium Azide
Applicable Applications for anti-Phospo-APC6 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Immunogen
The antibody was produced against synthesized peptide corresponding to amino acids 575-588 of Human APC6 protein.
Preparation and Storage
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot using Anti-Phospho-APC6 (Thr580) antibody (1:1,000) shows detection of a band ~72 kDa corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate. Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35ug of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose.)
Western Blot (WB)
(Sample Data: Western blot using Anti-Phospho-APC6 (Thr580) antibody (1:1,000) shows detection of a band ~72 kDa corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate. Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35ug of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then transferred to nitrocellulose.)
Related Product Information for anti-Phospo-APC6 antibody
APC6 (also known as Cell division cycle protein 16 homolog (CDC16Hs), anaphase promoting complex subunit 6, and cyclosome subunit 6) is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C is composed of at least 11 subunits. APC6 interacts with PPP5C and CDC20. This protein colocalizes with CDC27 to the centrosome at all stages of the cell cycle and to the mitotic spindle. Splice variants have been described for this protein.
Product Categories/Family for anti-Phospo-APC6 antibody
References
1. Tugendreich,S. et al.: CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition. Cell. 1995: 81 (2); 261-268.
2. Ollendorff,V. et al.: The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex. J. Biol. Chem. 1997: 272 (51); 32011-32018.
3. Kallio,M. et al.: Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events. J. Cell Biol. 1998: 141 (6); 1393-1406.
2. Ollendorff,V. et al.: The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex. J. Biol. Chem. 1997: 272 (51); 32011-32018.
3. Kallio,M. et al.: Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events. J. Cell Biol. 1998: 141 (6); 1393-1406.
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Product Notes
The Phospo-APC6 (Catalog #AAA515850) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospo-APC6 (Thr580) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phospo-APC6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). Researchers should empirically determine the suitability of the Phospo-APC6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospo-APC6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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