Western Blot (WB)
(Sample Data: Western blot using Anti-Phospho-APC1 (Ser377) (1:1,000) shows detection of a band ~215 kDa corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Each lane contains approximately 30 ug of HeLa whole cell lysates, separated by 4-8% SDS-PAGE followed by transfer to nitrocellulose.)
Rabbit anti-Human APC1 Polyclonal Antibody | anti-Phospho-APC1 antibody
Anti-Phospho-APC1 (Ser377)
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Immunoaffinity chromatography followed by solid phase adsorption.
Synonyms
APC1; Polyclonal Antibody; Anti-Phospho-APC1 (Ser377); anti-Phospho-APC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the APC1 protein phosphorylated at Serine 377
Purity/Purification
Immunoaffinity chromatography followed by solid phase adsorption.
Form/Format
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH7.2 + 0.01% (w/v) Sodium Azide
Applicable Applications for anti-Phospho-APC1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Immunogen
The antibody was produced against synthesized peptide corresponding to amino acids 373-382 of human APC1 protein.
Preparation and Storage
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot using Anti-Phospho-APC1 (Ser377) (1:1,000) shows detection of a band ~215 kDa corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Each lane contains approximately 30 ug of HeLa whole cell lysates, separated by 4-8% SDS-PAGE followed by transfer to nitrocellulose.)
Western Blot (WB)
(Sample Data: Western blot using Anti-Phospho-APC1 (Ser377) (1:1,000) shows detection of a band ~215 kDa corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Each lane contains approximately 30 ug of HeLa whole cell lysates, separated by 4-8% SDS-PAGE followed by transfer to nitrocellulose.)
Immunohistochemistry (IHC)
(Sample Data: Anti-Phospho-APC1 (Ser377) antibody was used at 5.0 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides at 60X. Tissue was formalin-fixed and paraffin embedded.)
Immunohistochemistry (IHC)
(Sample Data: Anti-Phospho-APC1 (Ser377) antibody was used at 5.0 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides at 60X. Tissue was formalin-fixed and paraffin embedded.)
Related Product Information for anti-Phospho-APC1 antibody
APC1 (also known as Anaphase promoting complex subunit 1, Cyclosome subunit 1, Protein Tsg24, Mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.
Product Categories/Family for anti-Phospho-APC1 antibody
References
1. Jorgensen,P.M., et al.: Characterization of the human APC1, the largest subunit of the anaphase-promoting complex. Gene. 2001;262 (1-2): 51-59.
2. Kraft C, et al.: Mitotic regulation of the human anaphase-promoting complex by phosphorylation. Embo J. 2003;22(24):6598-609
2. Kraft C, et al.: Mitotic regulation of the human anaphase-promoting complex by phosphorylation. Embo J. 2003;22(24):6598-609
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Product Notes
The Phospho-APC1 (Catalog #AAA515841) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospho-APC1 (Ser377) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's APC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the Phospho-APC1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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