Western Blot (WB)
(Sample Data: Western blot using Anti-p27KIP1 (CDKN1B) (1:500 dilution) shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead).)
anti-Human p27KIP1 (CDKN1B) Polyclonal Antibody | anti-p27KIP1 antibody
Anti-p27KIP1 (CDKN1B)
Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Purity
Delipidation and defibrination
Synonyms
p27KIP1 (CDKN1B); Polyclonal Antibody; Anti-p27KIP1 (CDKN1B); anti-p27KIP1 antibody
Host
Rabbit, Antiserum
Reactivity
Human
Clonality
Polyclonal
Specificity
Recognizes the p27 protein from human tissue
Purity/Purification
Delipidation and defibrination
Form/Format
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH7.2 + 0.01% (w/v) Sodium Azide
Applicable Applications for anti-p27KIP1 antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Immunogen
The antibody was produced against full length recombinant human p27 protein
Preparation and Storage
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot using Anti-p27KIP1 (CDKN1B) (1:500 dilution) shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead).)
Western Blot (WB)
(Sample Data: Western blot using Anti-p27KIP1 (CDKN1B) (1:500 dilution) shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead).)
Related Product Information for anti-p27KIP1 antibody
KIP1 (cyclin-dependent kinase inhibitor 1B) is a kinesin-related motor protein required for mitotic spindle assembly and chromosome segregation (1). Many tumorigenic processes modulate cell-cycle progression by regulating the levels of the cyclin-dependent kinase inhibitor KIP1. KIP1 binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (2). The phosphorylation-and ubiquitination-dependent proteolysis of KIP1 is implicated in control of the G1-S transition in the cell cycle. KIP1 is critical for retinoblastoma protein (Rb)-induced cellular proliferative senescence.
Product Categories/Family for anti-p27KIP1 antibody
References
1. Hoyt, M A. et al: Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly. J. Cell Biol. 1992 118(1):109-120.
2. Carrano,A C. et al: SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27. Nat. Cell Biol. 1999; 1, 193-199
2. Carrano,A C. et al: SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27. Nat. Cell Biol. 1999; 1, 193-199
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Product Notes
The p27KIP1 (Catalog #AAA515897) is an Antibody produced from Rabbit, Antiserum and is intended for research purposes only. The product is available for immediate purchase. The Anti-p27KIP1 (CDKN1B) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p27KIP1 (CDKN1B) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the p27KIP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p27KIP1 (CDKN1B), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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