Western Blot (WB)
(Sample Data: Western blot using Affinity Purified anti-Phospho-APC3 (Thr244) (1:500) to detect phosphorylated human CDC27 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Each lane contains approximately 30ug of HeLa whole cell lysates.)
Rabbit anti-Human APC3 Polyclonal Antibody | anti-Phospho-APC3 antibody
Anti-Phospho-APC3 (Thr 244)
Reactivity
Human
Applications
ELISA, Immunoprecipitation, Western Blot
Purity
Immunoaffinity chromatography
Synonyms
APC3; Polyclonal Antibody; Anti-Phospho-APC3 (Thr 244); anti-Phospho-APC3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the Cdc27 protein phosphorylated at Threonine 244
Purity/Purification
Immunoaffinity chromatography
Form/Format
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH7.2 + 0.01% (w/v) Sodium Azide
Applicable Applications for anti-Phospho-APC3 antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Immunogen
The antibody was produced against synthesized peptide corresponding to amino acids 239-240 of Human Cdc27 protein.
Preparation and Storage
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot using Affinity Purified anti-Phospho-APC3 (Thr244) (1:500) to detect phosphorylated human CDC27 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Each lane contains approximately 30ug of HeLa whole cell lysates.)
Western Blot (WB)
(Sample Data: Western blot using Affinity Purified anti-Phospho-APC3 (Thr244) (1:500) to detect phosphorylated human CDC27 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with nocodazole. Each lane contains approximately 30ug of HeLa whole cell lysates.)
Related Product Information for anti-Phospho-APC3 antibody
Cdc27 (also known as cell division cycle protein 27 homologue, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. Cdc27 is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Cdc27 has a nuclear localization.
Product Categories/Family for anti-Phospho-APC3 antibody
References
1. Gabellini,D., et al.: Early mitotic degradation of the homeoprotein HOXC10 is potentially linked to cell cycle progression. EMBO J. 2003: 22 (14); 3715-3724.
2. Topper,L.M., et al. The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis. Cell Cycle. 2002:1 (4); 282-292.
3. Wassmann,K., et al.: Mad2 transiently associates with an APC/p55Cdc complex during mitosis. Proc. Natl. Acad. Sci. U.S.A. 1988: 95 (19); 11193-11198.
2. Topper,L.M., et al. The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis. Cell Cycle. 2002:1 (4); 282-292.
3. Wassmann,K., et al.: Mad2 transiently associates with an APC/p55Cdc complex during mitosis. Proc. Natl. Acad. Sci. U.S.A. 1988: 95 (19); 11193-11198.
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Product Notes
The Phospho-APC3 (Catalog #AAA515846) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospho-APC3 (Thr 244) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's APC3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB). Researchers should empirically determine the suitability of the Phospho-APC3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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