Amphiphysin Recombinant Protein | AMPH recombinant protein

Purified Recombinant Amphiphysin protein (His tag) (GST tag)

Cat. Num. AAA5308512

• Gene Names: AMPH; AMPH1
• Applications: SDS-Page, Western Blot, ELISA
• Purity: > 90% pure
• Synonyms: Amphiphysin; Purified Recombinant Amphiphysin protein (His tag) (GST tag); Amphiphysin protein; AMPH protein; AMPH1 Protein; AMPH recombinant protein

• Host: Human
• Purity/Purification: > 90% pure
• Form/Format: Liquid in 50 mM Tris buffer, pH 8.0, 150 mM NaCL, 10% glycerol, 1 mM EDTA and 2 mM DTT
• Concentration: 1.0mg/ml (varies by lot)
• Sequence Length: 1-695 aa

• Applicable Applications for AMPH recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA)
• Expression: E Coli
• Protein Type: Recombinant
• Tag: His tag
• Residues: a-695 aa
• Product Use: Purified Recombinant Amphiphysin protein for use in SDS-PAGE and Western Blot.
• Biological Significance: Amphiphysin is a protein associated with the cytoplasmic surface of synaptic vesicles. Amphiphysin is a brain-enriched protein with an N-terminal lipid interaction, dimerisation and membrane bending BAR domain, a middle clathrin and adaptor binding domain and a C-terminal SH3 domain. In the brain, its primary function is thought to be the recruitment of dynamin to sites of clathrin-mediated endocytosis.
• Implications In Disease: Antibodies against AMPH are detected in patients with Stiff Person Syndrome (SPS), a rare disease of the central nervous system characterized by progressive rigidity of the body musculature with superimposed painful spasms. A subset of patients with SPS who were also affected by breast cancer are positive for autoantibodies against this protein.
• Sequence Information: Amphiphysin Is composed of 695 amino acids with a molecular weight of 109 kDa. There is a Phosphoserine residue at positions 506. Database Links Entrez Gene: 273 Human,
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Ships on dry ice. Upon receipt store at -70°C.

Western Blot (WB)

Product Categories/Family for AMPH recombinant protein

Neuroscience; Conjugated Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 550450
• NCBI GeneID: 273
• Molecular Weight: 109 kDa
• NCBI Official Full Name: amphiphysin
• NCBI Official Synonym Full Names: amphiphysin
• NCBI Official Symbol: AMPH
• NCBI Official Synonym Symbols: AMPH1
• NCBI Protein Information: amphiphysin
• UniProt Protein Name: Amphiphysin
• Protein Family: Amphiphysin
• UniProt Gene Name: AMPH
• UniProt Synonym Gene Names: AMPH1

NCBI Description

This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010]

Uniprot Description

amphiphysin: May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 7p14.1

Cellular Component: actin cytoskeleton; synaptic vesicle; trans-Golgi network

Molecular Function: ARF guanyl-nucleotide exchange factor activity; phospholipid binding; protein binding

Biological Process: endocytosis; synaptic transmission