Rabbit Phe Polyclonal Antibody | anti-phe antibody

Phe, phosphorylated (Ser/Thr)

Cat. Num. AAA626353

• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Synonyms: Phe; Polyclonal Antibody; phosphorylated (Ser/Thr); Anti -Phe; anti-phe antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes phosphoserine or threonine in the context of tyrosine, tryptophan or phenylalanine at the -1 position or phenylalanine at the +1 position (Y/F/W)(S*/T*)F. Does not recognize the nonphosphorylated form of these motifs, nor does it recognize other phosphoserine or threonine-containing proteins and peptides. Species Crossreactivity: Expected to crossreact with all species.
• Purity/Purification: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-phe antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunoprecipitation.; Dilution: ELISA (Peptide): 1:1000; Western Blot: 1:1000; Immunoprecipitation: 1:100
• Immunogen: Synthetic phosphoserine/threonine-phenylalanine containing peptide.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of calyculin A treated A431 cells, using MBS626353.)

ELISA (EIA)

(MBS626353 ELISA: Signal to noise ratio of phospho- versus nonphospho-peptides in which the phosphorylation site is adjacent to phenylalanine, tyrosine or tryptophan. (T* and S* denote phosphorylated threonine and serine.))

Related Product Information for anti-phe antibody

A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3. These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine. RSK1, p70S6K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity.

Product Categories/Family for anti-phe antibody

Antibodies; Abs to Amino Acids

NCBI and Uniprot Product Information

• NCBI GI #: 113473910
• NCBI GeneID: 4241508
• NCBI Accession #: YP_718173.1
• NCBI GenBank Nucleotide #: NC_008308.1
• NCBI Official Full Name: putative phenol 2-monooxygenase
• NCBI Official Symbol: phe
• NCBI Protein Information: putative phenol 2-monooxygenase
• Protein Family: Phenoloxidase