Mouse anti-Mammal 14-3-3 Binding Motif Monoclonal Antibody | anti-YWHAB antibody

14-3-3 Binding Motif, phosphorylated (Ser)

Cat. Num. AAA603373

• Gene Names: YWHAB; HS1; GW128; YWHAA; KCIP-1
• Reactivity: Mammal
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Ascites; Ascites
• Synonyms: 14-3-3 Binding Motif; Monoclonal Antibody; phosphorylated (Ser); Anti -14-3-3 Binding Motif; anti-YWHAB antibody

• Host: Mouse
• Reactivity: Mammal
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3i2
• Specificity: Recognizes peptides/proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. By ELISA, recognizes a wide range of peptides containing this phosphorylated 14-3-3 binding motif in a manner that is phospho-specific and largely independent of the surrounding amino acid sequence. Weakly crossreacts with sequences containing phospho-Thr instead of phospho-Ser in this motif and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresponding nonphosphorylated sequences.
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-YWHAB antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunoprecipitation.; Dilution: Peptide ELISA: 1:1000; Western Blot: 1:4000; Immunoprecipitation: 1:20 (Denatured proteins).
• Immunogen: Synthetic phospho-(Ser) 14-3-3 binding motif peptides (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of calyculin A treated A431 cells, using MBS603373 (left) or 0014-33 (right).)

ELISA (EIA)

(0014-33 14-3-3 Binding Motif, phosphorylated (Ser) ELISA assay: Signal-to-noise ratio of phospho- versus nonphospho-14-3-3 binding motif peptides. (S* denotes phosphorylated serine.))

Related Product Information for anti-YWHAB antibody

The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (1-5). Biological regulation by 14-3-3 is mediated through phosphorylation-dependent protein-protein interactions (6). Two different phospho-Ser-containing motifs are found within nearly all known 14-3-3 binding proteins (7). Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25c, FKHRL1, PKC and c-Raf (5,7).

Product Categories/Family for anti-YWHAB antibody

Antibodies; Abs to 14-3-3

NCBI and Uniprot Product Information

• NCBI GI #: 21328448
• NCBI GeneID: 7529
• NCBI Accession #: NP_647539.1
• NCBI GenBank Nucleotide #: NM_139323.3
• UniProt Accession #: P31946; A8K9K2; E1P616
• Molecular Weight: 28,082 Da
• NCBI Official Full Name: 14-3-3 protein beta/alpha
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
• NCBI Official Symbol: YWHAB
• NCBI Official Synonym Symbols: HS1; GW128; YWHAA; KCIP-1
• NCBI Protein Information: 14-3-3 protein beta/alpha; 14-3-3 alpha; protein 1054; brain protein 14-3-3, beta isoform; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, alpha polypeptide
• UniProt Protein Name: 14-3-3 protein beta/alpha
• Protein Family: 14-3-3 protein
• UniProt Gene Name: YWHAB
• UniProt Synonym Gene Names: KCIP-1
• UniProt Entry Name: 1433B_HUMAN

NCBI Description

This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

14-3-3 beta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: focal adhesion; cytoplasmic vesicle membrane; membrane; transcriptional repressor complex; perinuclear region of cytoplasm; cytoplasm; melanosome; cytosol; nucleus

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; enzyme binding; histone deacetylase binding; protein complex binding; phosphoprotein binding; transcription corepressor activity; phosphoserine binding

Biological Process: positive regulation of catalytic activity; negative regulation of protein amino acid dephosphorylation; epidermal growth factor receptor signaling pathway; axon guidance; fibroblast growth factor receptor signaling pathway; activation of MAPKK activity; nerve growth factor receptor signaling pathway; protein heterooligomerization; apoptosis; MAPKKK cascade; small GTPase mediated signal transduction; Ras protein signal transduction; cytoplasmic sequestering of protein; insulin receptor signaling pathway; innate immune response; gene expression; negative regulation of transcription, DNA-dependent; vascular endothelial growth factor receptor signaling pathway; protein targeting