Testing Data
(Specificity and sensitivity of MBS615382. The antibody reacts specifically with as little as 0.25ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4ug of nonphosphorylated p42 MAP kinase.)
Rabbit MAP Kinase p44, p42 Polyclonal Antibody | anti-MAPK3 antibody
MAP Kinase p44, p42, phosphorylated (Thr202, Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Ki
Purified by Protein A and peptide affinity chromatography.
Purified by Protein A and peptide affinity chromatography.
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Immunofluorescence (IF-IC): 1:1000
Flow Cytometry: 1:100
Testing Data
(Specificity and sensitivity of MBS615382. The antibody reacts specifically with as little as 0.25ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4ug of nonphosphorylated p42 MAP kinase.)
Testing Data
(Specificity and sensitivity of MBS615382. The antibody reacts specifically with as little as 0.25ng of phosphorylated p42 MAP kinase and does not cross-react with up to 4ug of nonphosphorylated p42 MAP kinase.)
Testing Data
(Confocal images of double immunostaining of rat brain hippocampus (dentate gyrus region), control and 30 minutes of reperfusion following 15 minute transient cerebral ischemia, using MBS615382 (green) and microtubulin associated protein-2 (MAP-2, red). Yellow represents overlay of red and green. (Provided by Dr. Bingren Hu, University of Miami School of Medicine, Florida.))
Testing Data
(Confocal images of double immunostaining of rat brain hippocampus (dentate gyrus region), control and 30 minutes of reperfusion following 15 minute transient cerebral ischemia, using MBS615382 (green) and microtubulin associated protein-2 (MAP-2, red). Yellow represents overlay of red and green. (Provided by Dr. Bingren Hu, University of Miami School of Medicine, Florida.))
Testing Data
(Phosphorylated MEK and Erk were assayed in human peripheral blood lymphocytes stimulated with PMA in the presence or absence of the Raf inhibitor BAY 37-9751 or M2865-50 MEK1, 2 Inhibitor. BAY 37-951 blocked PMA-stimulated phosphorylation of both MEK and Erk, consistent with inhibition at the level of Raf, while U0126 blocked phosphorylation of Erk only, consistent with inhibition at the level of MEK. From Chow, S. et al. (2001) Cytometry 46, 72-78.)
Testing Data
(Phosphorylated MEK and Erk were assayed in human peripheral blood lymphocytes stimulated with PMA in the presence or absence of the Raf inhibitor BAY 37-9751 or M2865-50 MEK1, 2 Inhibitor. BAY 37-951 blocked PMA-stimulated phosphorylation of both MEK and Erk, consistent with inhibition at the level of Raf, while U0126 blocked phosphorylation of Erk only, consistent with inhibition at the level of MEK. From Chow, S. et al. (2001) Cytometry 46, 72-78.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Jurkat cells, untreated (green) or PMA-treated (blue), using MBS615382 compared to a nonspecific negative control antibody (red).)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Jurkat cells, untreated (green) or PMA-treated (blue), using MBS615382 compared to a nonspecific negative control antibody (red).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.
Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.24; Protein kinase, CMGC; CMGC group; MAPK family; ERK subfamily; MAPK/ERK subfamily
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: Golgi apparatus; focal adhesion; mitochondrion; early endosome; nuclear envelope; caveola; pseudopodium; cytosol; nucleoplasm; microtubule cytoskeleton; cytoskeleton; late endosome; nucleus
Molecular Function: MAP kinase activity; protein binding; phosphotyrosine binding; ATP binding; phosphatase binding
Biological Process: axon guidance; activation of MAPKK activity; nerve growth factor receptor signaling pathway; viral reproduction; DNA damage induced protein phosphorylation; positive regulation of histone phosphorylation; activation of MAPK activity; apoptosis; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; sensory perception of pain; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; BMP signaling pathway; toll-like receptor 5 signaling pathway; response to exogenous dsRNA; regulation of transcription factor activity; small GTPase mediated signal transduction; lipopolysaccharide-mediated signaling pathway; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; transcription from RNA polymerase I promoter; cell cycle; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of stress-activated MAPK cascade; organ morphogenesis; cartilage development; Ras protein signal transduction; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; gene expression; positive regulation of histone acetylation; toll-like receptor 9 signaling pathway; positive regulation of protein amino acid phosphorylation; transcription initiation from RNA polymerase I promoter; blood coagulation; vascular endothelial growth factor receptor signaling pathway; phosphorylation; regulation of cytoskeleton organization and biogenesis
Research Articles on MAPK3
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Product Notes
The MAPK3 mapk3 (Catalog #AAA615382) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAP Kinase p44, p42, phosphorylated (Thr202, Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Ki reacts with Bovine, Chicken, Hamster, Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAP Kinase p44, p42 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunoprecipitation: 1:50 Immunofluorescence (IF-IC): 1:1000 Flow Cytometry: 1:100. Researchers should empirically determine the suitability of the MAPK3 mapk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP Kinase p44, p42, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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