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Western Blot (WB)
(Anti-PGRMC1 rabbit polyclonal antibody at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Hela Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:24 kDa)
Rabbit anti-Human PGRMC1 Polyclonal Antibody | anti-PGRMC1 antibody
Anti-PGRMC1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
PGRMC1; Polyclonal Antibody; Anti-PGRMC1 Antibody; Rabbit Polyclonal; PGRMC1 Antibody; Rabbit PAb; Antigen Affinity Purified; progesterone receptor membrane component 1; Anti-HPR6.6 Antibody; Anti-MPR Antibody; anti-PGRMC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PGRMC1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PGRMC1 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:200
IP: 1-5uL/mg of lysate
IHC-P: 1:50-1:200
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human PGRMC1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PGRMC1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PGRMC1 rabbit polyclonal antibody at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Hela Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:24 kDa)
Western Blot (WB)
(Anti-PGRMC1 rabbit polyclonal antibody at 1:500 dilution Lane A: MCF7 Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Hela Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:22 kDa Observed band size:24 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human PGRMC1 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PGRMC1 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PGRMC1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human PGRMC1 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(PGRMC1 was immunoprecipitated using: Lane A:0.5 mg MCF7 Whole Cell Lysate 4 uL anti-PGRMC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PGRMC1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 22 kDa Observed band size :25 kDa)
Immunoprecipitation (IP)
(PGRMC1 was immunoprecipitated using: Lane A:0.5 mg MCF7 Whole Cell Lysate 4 uL anti-PGRMC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PGRMC1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 22 kDa Observed band size :25 kDa)
Related Product Information for anti-PGRMC1 antibody
Progesterone Receptor Membrane Component 1 (PGRMC1) is located in many different subcellular locations with many different attested and probably location-specific functions. PGRMC1 is a highly conserved multifunctional protein that is found in numerous systems, including the reproductive system. Interestingly, PGRMC1 is expressed at several intracellular locations, including the nucleolus. PGRMC1 enhances autophagy, activates cytochrome p450, and inveigles signaling pathways to promote cell survival and reduce the effect of drug treatments. PGRMC1 was identified in the mitochondrial outer membrane where it interacts with ferrochelatase, the last enzyme in the heme synthetic pathway. It has been proposed that PGRMC1 may act as a chaperone to shuttle newly synthesized heme from the mitochondrion to cytochrome P450 (cyP450) enzymes.
References
Zhu X, et al. (2017) Pgrmc1-dependent autophagy by hyperoside induces apoptosis and sensitizes ovarian cancer cells to cisplatin treatment. Int J Oncol 50 (3): 835-846.Terzaghi L, et al. (2018) Pgrmc1 localization and putative function in the nucleolus of bovine granulosa cells and oocytes. Reproduction 155 (3): 273-282.Cahill MA, et al. (2017) Thoughts on interactions between pgrmc1 and diverse attested and potential hydrophobic ligands. J Steroid Biochem Mol Biol 171 11-33.Cahill MA, et al. (2016) Pgrmc1 regulation by phosphorylation: Potential new insights in controlling biological activity. Oncotarget 7 (32): 50822-50827.
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Product Notes
The PGRMC1 (Catalog #AAA8108909) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PGRMC1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PGRMC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:50-1:200 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the PGRMC1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PGRMC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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