Rabbit anti-Human PEX3 Polyclonal Antibody | anti-PEX3 antibody

Anti-PEX3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108003

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PEX3; Polyclonal Antibody; Anti-PEX3 Antibody; Rabbit Polyclonal; PEX3 Antibody; Rabbit PAb; Antigen Affinity Purified; peroxisomal biogenesis factor 3; Anti-PBD10A Antibody; Anti-TRG18 Antibody; anti-PEX3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PEX3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PEX3 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human PEX3 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human PEX3 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PEX3 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:42 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human PEX3 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(PEX3 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-PEX3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PEX3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 42 kDa Observed band size :43 kDa)

Related Product Information for anti-PEX3 antibody

PEX3 is a peroxisomal membrane protein (PMP) that functions in the import of PMPs into the peroxisomal membrane and has been shown to interact with pexophagic receptor proteins during pexophagy in yeast. Thus, PEX3 is important not only for peroxisome biogenesis but also for peroxisome degradation. Pex3 is an evolutionarily conserved type III peroxisomal membrane protein required for peroxisome formation. It is inserted into the ER membrane and sorted via an ER subdomain (the peroxisomal ER, or pER) to peroxisomes. The human peroxins PEX3 and PEX19 play a central role in peroxisomal membrane biogenesis. The membrane-anchored PEX3 serves as the receptor for cytosolic PEX19, which in turn recognizes newly synthesized peroxisomal membrane proteins. After delivering these proteins to the peroxisomal membrane, PEX19 is recycled to the cytosol.

References

Yamashita S, et al. (2014) The membrane peroxin pex3 induces peroxisome-ubiquitination-linked pexophagy. Autophagy 10 (9): 1549-1564.Soukupova M, et al. (1999) Identification and characterization of the human peroxin pex3. Eur J Cell Biol 78 (6): 357-374.Fakieh MH, et al. (2013) Intra-er sorting of the peroxisomal membrane protein pex3 relies on its luminal domain. Biol Open 2 (8): 829-837.