Western Blot (WB)
(Anti-UBE2J1 rabbit polyclonal antibody at 1:2000 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:40 kDa)
Rabbit anti-Human UBC6e/UBE2J1 Polyclonal Antibody | anti-Ube2j1 antibody
Anti-UBC6e/UBE2J1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
UBC6e/UBE2J1; Polyclonal Antibody; Anti-UBC6e/UBE2J1 Antibody; Rabbit Polyclonal; UBC6e/UBE2J1 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-UBC6e Antibody; anti-Ube2j1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human UBC6e/UBE2J1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-Ube2j1 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:2000-1:10,000
IP: 1-5uL/mg of lysate
IP: 1-5uL/mg of lysate
Immunogen
E Coli-derived Human UBC6e/UBE2J1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human UBC6e/UBE2J1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-UBE2J1 rabbit polyclonal antibody at 1:2000 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:40 kDa)
Western Blot (WB)
(Anti-UBE2J1 rabbit polyclonal antibody at 1:2000 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:35 kDa Observed band size:40 kDa)
Immunoprecipitation (IP)
(UBE2J1 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-UBE2J1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-UBE2J1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa Observed band size :36 kDa)
Immunoprecipitation (IP)
(UBE2J1 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-UBE2J1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-UBE2J1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 36 kDa Observed band size :36 kDa)
Related Product Information for anti-Ube2j1 antibody
Ubiquitin-mediated protein degradation is involved in many cellular processes, including the degradation of unfolded or misfolded proteins in ER through the pathway called endoplasmic reticulum-associated protein degradation (ERAD). To be degraded in proteasomes, first ERAD substrates must be retro-translocated to the cytosol with the involvement of chaperones and components of the protein translocon machinery. Ubiquitination enzymes that are localized at the cytosolic face of the ER membrane then target ERAD substrate for proteasomal degradation. There are only three E2s: Ube2j1, Ube2j2, and Ube2G2 that are implicated in the degradation of ERAD substrates. Ube2j1 and Ube2j2 are the only ER resident E2s which has a short luminal region, a single transmembrane domain and catalytic domain that is oriented toward the cytosol. Ube2G2 lacks both luminal and transmembrane domain and it functions in ERAD from the cytosol.
References
Elangovan M, et al. (2017) The role of ubiquitin-conjugating enzyme ube2j1 phosphorylation and its degradation by proteasome during endoplasmic stress recovery. J Cell Commun Signal 11 (3): 265-273.
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Product Notes
The Ube2j1 (Catalog #AAA8109568) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-UBC6e/UBE2J1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's UBC6e/UBE2J1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:2000-1:10,000 IP: 1-5uL/mg of lysate. Researchers should empirically determine the suitability of the Ube2j1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBC6e/UBE2J1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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