Rabbit anti-Human PCMT1 Polyclonal Antibody | anti-PCMT1 antibody

PCMT1 Antibody (Center)

Cat. Num. AAA9217330

• Gene Names: PCMT1; PIMT
• Reactivity: Human
• Applications: Western Blot, ELISA
• Synonyms: PCMT1; Polyclonal Antibody; PCMT1 Antibody (Center); Protein-L-isoaspartate(D-aspartate) O-methyltransferase; PIMT; 2.1.1.77; L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl/D-aspartyl methyltransferase; Protein-beta-aspartate methyltransferase; Purified Rabbit Polyclonal Antibody (Pab); anti-PCMT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This PCMT1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 93-126 amino acids from the Central region of human PCMT1.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 1.71 (varies by lot)
• Sequence Length: 227

• Applicable Applications for anti-PCMT1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB~~1:2000
• Cellular Location: Cytoplasm.
• Antigen Type: Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-PCMT1 Antibody (Center) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: HepG2 whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Raji whole cell lysateLane 5: 293 whole cell lysateLane 6: 293T/17 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-PCMT1 antibody

Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl- terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein (By similarity).

Product Categories/Family for anti-PCMT1 antibody

Cell Biology

References

Ingrosso D.,et al.J. Biol. Chem. 264:20131-20139(1989). Maclaren D.C.,et al.Biochem. Biophys. Res. Commun. 185:277-283(1992). Takeda R.,et al.J. Biochem. 117:683-685(1995). Shirasawa T.,et al.Submitted (APR-1994) to the EMBL/GenBank/DDBJ databases. Ota T.,et al.Nat. Genet. 36:40-45(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 317373537
• NCBI GeneID: 5110
• UniProt Accession #: P22061; Q14661; Q16556; Q5VYC1; Q5VYC2; Q93061; Q96II9; Q99625; Q9BQV7; Q9BQV8; A8K109; J3KP72
• Molecular Weight: 24636 Da
• NCBI Official Full Name: Protein-L-isoaspartate(D-aspartate) O-methyltransferase
• NCBI Official Synonym Full Names: protein-L-isoaspartate (D-aspartate) O-methyltransferase
• NCBI Official Symbol: PCMT1
• NCBI Official Synonym Symbols: PIMT
• NCBI Protein Information: protein-L-isoaspartate(D-aspartate) O-methyltransferase
• UniProt Protein Name: Protein-L-isoaspartate(D-aspartate) O-methyltransferase
• UniProt Gene Name: PCMT1
• UniProt Synonym Gene Names: PIMT
• UniProt Entry Name: PIMT_HUMAN

NCBI Description

This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

PCMT1: Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl- terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein. Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.77; Methyltransferase

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum

Molecular Function: protein binding; protein-L-isoaspartate (D-aspartate) O-methyltransferase activity

Biological Process: protein amino acid methylation; protein repair