anti-Human CATD Antibody | anti-CATD antibody

CATD (heavy chain, Cleaved-Leu169) Antibody

Cat. Num. AAA851762

• Gene Names: CTSD; CPSD; CLN10; HEL-S-130P
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: CATD; CATD (heavy chain; Cleaved-Leu169) Antibody; Cathepsin D [Precursor]; EC 3.4.23.5; Cathepsin D light chain; Cathepsin D heavy chain; CTSD; CPSD; anti-CATD antibody

• Reactivity: Human
• Specificity: CATD (heavy chain, Cleaved-Leu169) antibody detects endogenous levels of fragment of activated CATD resulting from cleavage adjacent to Leu169
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 412

• Applicable Applications for anti-CATD antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:40000
• Immunogen: The antiserum was produced against synthesized peptide derived from human CATD.
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells and NIH-3T3 cells treated with etoposide (25uM, 1hour), Jurkat cells and A549 cells treated with etoposide (25uM, 24hours), using CATD (heavy chain, Cleaved-Leu169) antibody.)

References

Faust P.L., Proc. Natl. Acad. Sci. U.S.A. 82:4910-4914(1985). Westley B.R., Nucleic Acids Res. 15:3773-3786(1987). Redecker B., DNA Cell Biol. 10:423-431(1991).

NCBI and Uniprot Product Information

• NCBI GI #: 4503143
• NCBI GeneID: 1509
• NCBI Accession #: NP_001900.1
• NCBI GenBank Nucleotide #: NM_001909.4
• UniProt Accession #: P07339; Q6IB57
• Molecular Weight: 44,552 Da
• NCBI Official Full Name: cathepsin D preproprotein
• NCBI Official Synonym Full Names: cathepsin D
• NCBI Official Symbol: CTSD
• NCBI Official Synonym Symbols: CPSD; CLN10; HEL-S-130P
• NCBI Protein Information: cathepsin D
• UniProt Protein Name: Cathepsin D
• Protein Family: Putative oxidoreductase
• UniProt Gene Name: CTSD
• UniProt Synonym Gene Names: CPSD

NCBI Description

This gene encodes a member of the A1 family of peptidases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the cathepsin D light and heavy chains, which heterodimerize to form the mature enzyme. This enzyme exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease. [provided by RefSeq, Nov 2015]

Uniprot Description

Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation (PubMed:27333034). Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.