Rabbit PCMT1 Polyclonal Antibody | anti-PCMT1 antibody

PCMT1 Rabbit pAb

Cat. Num. AAA4758991

• Gene Names: PCMT1; PIMT
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: PCMT1; Polyclonal Antibody; PCMT1 Rabbit pAb; protein-L-isoaspartate (D-aspartate) O-methyltransferase; PIMT; anti-PCMT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purified
• Form/Format: pH7.4 PBS, 0.05% NaN3, 40% Glycerol
• Concentration: 1.2mg/ml (varies by lot)

• Applicable Applications for anti-PCMT1 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:1000-1:5000; IHC-P: 1:200-1:300
• Immunogen: Fusion protein of human PCMT1
• Conjugation: Unconjugated
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western Blot analysis of PCMT1 in Jurkat, Raji, 231, HepG2, Hela and 293T cell lysates in order using PCMT1 Rabbit pAb at dilution 1/1000)

Immunohistochemistry (IHC)

(Immunohistochemistry of PCMT1 in paraffin-embedded Human esophagus cancer tissue using PCMT1 Rabbit pAb at dilution 1/160. Predicted cell location: Cytoplasm)

Related Product Information for anti-PCMT1 antibody

This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

NCBI and Uniprot Product Information

• NCBI GI #: 354983493
• NCBI GeneID: 5110
• NCBI Accession #: NP_001238978.1
• NCBI GenBank Nucleotide #: NM_001252049.1
• UniProt Accession #: P22061; Q14661; Q16556; Q5VYC1; Q5VYC2; Q93061; Q96II9; Q99625; Q9BQV7; Q9BQV8; A8K109; J3KP72
• Molecular Weight: 24,679 Da
• NCBI Official Full Name: protein-L-isoaspartate(D-aspartate) O-methyltransferase isoform 2
• NCBI Official Synonym Full Names: protein-L-isoaspartate (D-aspartate) O-methyltransferase
• NCBI Official Symbol: PCMT1
• NCBI Official Synonym Symbols: PIMT
• NCBI Protein Information: protein-L-isoaspartate(D-aspartate) O-methyltransferase; protein-L-isoaspartate(D-aspartate) O-methyltransferase; L-isoaspartyl protein carboxyl methyltransferase; protein L-isoaspartyl/D-aspartyl methyltransferase; protein-beta-aspartate methyltransferas
• UniProt Protein Name: Protein-L-isoaspartate(D-aspartate) O-methyltransferase
• UniProt Gene Name: PCMT1
• UniProt Synonym Gene Names: PIMT
• UniProt Entry Name: PIMT_HUMAN

NCBI Description

This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

PCMT1: Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl- terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein. Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.77; Methyltransferase

Chromosomal Location of Human Ortholog: 6q25.1

Cellular Component: endoplasmic reticulum; cytoplasm; vesicle

Molecular Function: protein-L-isoaspartate (D-aspartate) O-methyltransferase activity

Biological Process: protein repair; protein amino acid methylation