PCMT recombinant protein

PCMT, 1-227aa, Human, His tag, E Coli

Cat. Num. AAA203718

• Gene Names: PCMT1; PIMT
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: PCMT; 1-227aa; Human; His tag; E Coli; Protein-L-isoaspartate O-methyltransferase; Protein-L-isoaspartate O-methyltransferase L isoaspartyl protein carboxyl methyltransferase; L isoaspartyl/D aspartyl methyltransferase; PCMT 1; PIMT; Protein beta aspartate methyltransferase; Protein L isoaspartate (D aspartate) O methyltransferase; Protein L isoaspartyl/D aspartyl methyltransferase; ; PCMT recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 0.1M NaCl
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAWK SGGASHSELI HNLRKNGIIK TDKVFEVMLA TDRSHYAKCN PYMDSPQSIG FQATISAPHM HAYALELLFD QLHEGAKALD VGSGSGILTA CFARMVGCTG KVIGIDHIKE LVDDSINNVR KDDPTLLSSG RVQLVVGDGR MGYAEEAPYD AIHVGAAAPV VPQALIDQLK PGGRLILPVG PAGGNQMLEQ YDKLQDGSIK MKPLMGVIYV PLTDKEKQWS RWK
• Sequence Length: 227

• Applicable Applications for PCMT recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PCMT recombinant protein

PCMT1, also known as L-isoaspartyl protein carboxyl methyltransferase, is an enzyme that catalyses the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. This protein plays a role in the repair and/or degradation of damaged proteins. Recombinant human PCMT1 protien, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PCMT recombinant protein

Enzymes & Proteases

References

Kagan RM., et al. (1997) Comp Biochem Physiol B Biochem Mol Biol. 117 (3):379-85.

NCBI and Uniprot Product Information

• NCBI GI #: 14250587
• NCBI GeneID: 5110
• Molecular Weight: 28.8 kDa (263aa) confirmed by MALDI-TOF
• NCBI Official Full Name: Protein-L-isoaspartate (D-aspartate) O-methyltransferase
• NCBI Official Synonym Full Names: protein-L-isoaspartate (D-aspartate) O-methyltransferase
• NCBI Official Symbol: PCMT1
• NCBI Official Synonym Symbols: PIMT
• NCBI Protein Information: protein-L-isoaspartate(D-aspartate) O-methyltransferase
• UniProt Protein Name: Protein-L-isoaspartate(D-aspartate) O-methyltransferase
• Protein Family: Protein-L-isoaspartate(D-aspartate) O-methyltransferase
• UniProt Gene Name: PCMT1
• UniProt Synonym Gene Names: PIMT

NCBI Description

This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.