Western Blot (WB)
(Western blot analysis of p53AIP in HL60 cell lysate with MBS610087 at (A) 4 and (B) 8ug/ml.)
Rabbit anti-Human, Mouse p53 AIP-1 Polyclonal Antibody
p53 AIP-1 (p53-regulated Apoptosis Inducing Protein 1)
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
p53 AIP-1; Polyclonal Antibody; p53 AIP-1 (p53-regulated Apoptosis Inducing Protein 1); Anti -p53 AIP-1 (p53-regulated Apoptosis Inducing Protein 1); anti-p53 AIP-1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human p53AIP. Will detect all three isoforms.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.02% sodium azide.
Applicable Applications for anti-p53 AIP-1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 4-8ug/ml. See P1001-32V4 for control peptide.
Dilution: Western Blot: 4-8ug/ml. See P1001-32V4 for control peptide.
Immunogen
15 amino acid peptide from near the amino terminus of human p53AIP (GenBank accession no. BAB16421)
Positive Control
HL60 cell lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of p53AIP in HL60 cell lysate with MBS610087 at (A) 4 and (B) 8ug/ml.)
Western Blot (WB)
(Western blot analysis of p53AIP in HL60 cell lysate with MBS610087 at (A) 4 and (B) 8ug/ml.)
Related Product Information for anti-p53 AIP-1 antibody
The p53 tumor-suppressor protein can induce apoptosis through transcriptional activation of several genes. One such protein p53AIP was initially identified through direct cloning of p53 binding sequences from human genomic DNA. Its expression is inducible by p53 following p53 phosphorylation on Ser-46, and ectopic expression of p53AIP leads to apoptotic cell death. Both the phosphorylation of p53 and the induction of p53AIP were blocked by inhibiting the expression of p53DINP1 by the introduction of antisense oligonucleotides to p53DINP1, suggesting that the apoptosis associated with p53AIP expression is regulated by p53DINP1. Finally, as adenovirus-mediated introduction of p53AIP has been shown to suppress tumor growth in vivo, it has been suggested that p53AIP gene transfer may become a useful strategy for the treatment of p53-resistant cancers. Three isoforms of p53AIP are known to exist; this antibody will detect all three.
Product Categories/Family for anti-p53 AIP-1 antibody
Similar Products
Product Notes
The p53 AIP-1 (Catalog #AAA610087) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p53 AIP-1 (p53-regulated Apoptosis Inducing Protein 1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's p53 AIP-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 4-8ug/ml. See P1001-32V4 for control peptide. Researchers should empirically determine the suitability of the p53 AIP-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p53 AIP-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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