Rabbit Kangai 1 Polyclonal Antibody | anti-CD82 antibody

Kangai 1, CT (KAI1, CD82, R2 Leukocyte Antigen, Suppression of Tumorigenicity 6)

Cat. Num. AAA618705

• Gene Names: CD82; R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Kangai 1; Polyclonal Antibody; CT (KAI1; CD82; R2 Leukocyte Antigen; Suppression of Tumorigenicity 6); Anti -Kangai 1; anti-CD82 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KAI1. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-CD82 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5-1ug/ml. See K0003-50A for control peptide.
• Immunogen: 15 amino acid peptide from near the carboxy terminus of human KAI1 (GenBank accession no. NP_002222)
• Positive Control: A549 cell lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of KAI1 in A549 cell lysate with KAI1 antibody at (A) 0.5 and (B) 1ug/ml.)

Related Product Information for anti-CD82 antibody

KAI1 was initially identified from a T-cell activation study as a four-transmembrane protein that plays an accessory role in T-cell activation (1,2), and was later determined to act as a cancer metastasis suppressor gene (3). This protein is ubiquitously expressed at moderate to high levels in most tissues, but its expression is downregulated during tumor progression. The loss of KAI1 and p53 is associated with poor survival for prostate and other cancer patients (4). Recently, KAI1 was found to interact with DARC, the Duffy antigen for chemokines using a yeast two hybrid screen (5). It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases. At least two isoforms of KAI1 are known to exist.

Product Categories/Family for anti-CD82 antibody

Antibodies; Abs to CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 32351053
• NCBI GeneID: 3732
• UniProt Accession #: Q7Z5N2; P27701; Q6LET0
• Molecular Weight: 26,788 Da
• NCBI Official Full Name: kangai 1
• NCBI Official Synonym Full Names: CD82 molecule
• NCBI Official Symbol: CD82
• NCBI Official Synonym Symbols: R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
• NCBI Protein Information: CD82 antigen; tspan-27; C33 antigen; tetraspanin-27; OTTHUMP00000232950; OTTHUMP00000232951; inducible membrane protein R2; metastasis suppressor Kangai-1; kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4))
• UniProt Protein Name: Kangai 1
• Protein Family: CD82 antigen
• UniProt Gene Name: KAI1
• UniProt Entry Name: Q7Z5N2_HUMAN

NCBI Description

This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

CD82: a widespread transmembrane protein of the tetraspanin family. A metastasis-suppressor whose decreased expression may be involved in malignant progression. Suppresses tumor metastasis of many cancers including cancers of the prostate, bladder, colon, cervix, liver, and lung (NSCLC). It is a target of estrogen receptor-mediated gene repression and is downregulated in primary human breast cancer. May be a prognostic marker for lung cancer and tumor metastatic potential. Interacts with cell surface proteins including integrins, cadherins, CD4, CD8, IGSF8. Modulates EGFR signaling. May suppress invasion by inhibiting integrin-dependent crosstalk with c-Met receptor and Src kinases. Its regulation of c-Met signaling apparently affects cancer cell migration.

Protein type: Cell surface; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Disease: Prostate Cancer