Immunohistochemistry (IHC)
(Human Placenta (formalin-fixed, paraffin-embedded) stained with ORAI3 antibody at 3ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Rabbit anti-Human ORAI3 Polyclonal Antibody | anti-Orai3 antibody
ORAI3, CT (Protein Orai-3, Transmembrane Protein 142C, TMEM142C)
Gene Names
Orai3; BC061259; Tmem142c; 9930124N15
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
ORAI3; Polyclonal Antibody; CT (Protein Orai-3; Transmembrane Protein 142C; TMEM142C); Anti -ORAI3; anti-Orai3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ORA13. Species sequence homology: mouse
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.02% sodium azide.
Applicable Applications for anti-Orai3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Dilution: Western Blot: 2-4ug/ml
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3ug/ml
Immunocytochemistry: 5ug/ml
Dilution: Western Blot: 2-4ug/ml
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3ug/ml
Immunocytochemistry: 5ug/ml
Immunogen
Synthetic peptide corresponding to 19aa from near the C-terminal of human ORAI3 (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Human Placenta (formalin-fixed, paraffin-embedded) stained with ORAI3 antibody at 3ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Immunohistochemistry (IHC)
(Human Placenta (formalin-fixed, paraffin-embedded) stained with ORAI3 antibody at 3ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Western Blot (WB)
(Western blot analysis of ORAI3 in A20 cell lysate with ORAI3 antibody at (A) 2 and (B) 4ug/ml.)
Western Blot (WB)
(Western blot analysis of ORAI3 in A20 cell lysate with ORAI3 antibody at (A) 2 and (B) 4ug/ml.)
Related Product Information for anti-Orai3 antibody
ORAI3, also known as Transmembrane protein 142C (TMEM142C), is a 31.5kD multi-pass membrane protein belonging to the ORAI family. Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI3 is one of two mammalian homologs of ORAI1, a four- transmembrane spanning protein that is an essential component of CRAC channels. ORAI3, along with ORAI1 and ORAI2, functions as a Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Studies indicate that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2.
Product Categories/Family for anti-Orai3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
31,358 Da
NCBI Official Full Name
Orai3
NCBI Official Synonym Full Names
ORAI calcium release-activated calcium modulator 3
NCBI Official Symbol
Orai3
NCBI Official Synonym Symbols
BC061259; Tmem142c; 9930124N15
NCBI Protein Information
protein orai-3; transmembrane protein 142C; calcium release-activated calcium channel 3
UniProt Protein Name
Protein orai-3
Protein Family
UniProt Gene Name
Orai3
UniProt Synonym Gene Names
Tmem142c
UniProt Entry Name
ORAI3_MOUSE
Uniprot Description
TMEM142C: Key regulator or component of store-operated Ca(2+) channel and transcription factor NFAT nuclear import. Belongs to the Orai family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Cellular Component: membrane; integral to membrane
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Product Notes
The Orai3 orai3 (Catalog #AAA6002919) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ORAI3, CT (Protein Orai-3, Transmembrane Protein 142C, TMEM142C) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ORAI3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Dilution: Western Blot: 2-4ug/ml Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3ug/ml Immunocytochemistry: 5ug/ml. Researchers should empirically determine the suitability of the Orai3 orai3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ORAI3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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