Rabbit anti-Mouse, Rat ORAI3 Polyclonal Antibody | anti-ORAI3 antibody

RABBIT ANTI ORAI3 (N-TERMINAL)

Cat. Num. AAA222947

• Gene Names: ORAI3; TMEM142C
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: ORAI3; Polyclonal Antibody; RABBIT ANTI ORAI3 (N-TERMINAL); anti-ORAI3 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises human ORAI3, also known as Transmembrane protein 142C (TMEM142C), a 31.5 kDa multi-pass membrane protein belonging to the ORAI family. ; ; Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ; ; ORAI3 is one of two mammalian homologs of ORAI1, a four- transmembrane spanning protein that is an essential component of CRAC channels. ORAI3, along with ORAI1 and ORAI2, functions as a Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Studies indicate that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. ; ; This item is not expected to cross react with either ORAI1 or ORAI2.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 295

• Applicable Applications for anti-ORAI3 antibody: Immunohistology Frozen, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 35kDa in A20 cell lysates.; Immunohistology - Frozen: Maximum Dilution: 10ug/ml; Western Blotting: Minimum Dilution: 1; Maximum Dilution: 2ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Immunogen: A 15 amino acid peptide from near the amino terminus of human ORAI3.; Buffer Solution
• Antiserum Preparation: Antiserum to human ORAI3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysate from A20 mouse B-cell lymphoma cells probed with Rabbit anti Human ORAI3 (MBS222947) at 1(A), 2(B) and 4(C) ug/ml)

Testing Data #2

(Immunocytochemical staining of A20 cells with Rabbit anti Human ORAI3 (MBS222947))

Related Product Information for anti-ORAI3 antibody

MBS222947 recognises human ORAI3, also known as Transmembrane protein 142C (TMEM142C), a 31.5 kDa multi-pass membrane protein belonging to the ORAI family. Antigen stimulation of immune cells triggers Ca2+ entry through Ca2+ release-activated Ca2+ (CRAC) channels. CRAC channels are the main pathway for Ca2+ influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT. ORAI3 is one of two mammalian homologs of ORAI1, a four- transmembrane spanning protein that is an essential component of CRAC channels. ORAI3, along with ORAI1 and ORAI2, functions as a Ca2+ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca2+ sensor. Studies indicate that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. MBS222947 is not expected to cross react with either ORAI1 or ORAI2.

NCBI and Uniprot Product Information

• NCBI GI #: 22748651
• NCBI GeneID: 93129
• NCBI Accession #: NP_689501.1
• NCBI GenBank Nucleotide #: NM_152288.2
• UniProt Accession #: Q9BRQ5; Q96BI8
• Molecular Weight: 31,499 Da
• NCBI Official Full Name: protein orai-3
• NCBI Official Synonym Full Names: ORAI calcium release-activated calcium modulator 3
• NCBI Official Symbol: ORAI3
• NCBI Official Synonym Symbols: TMEM142C
• NCBI Protein Information: protein orai-3; transmembrane protein 142C
• UniProt Protein Name: Protein orai-3
• Protein Family: Protein
• UniProt Gene Name: ORAI3
• UniProt Synonym Gene Names: TMEM142C
• UniProt Entry Name: ORAI3_HUMAN

Uniprot Description

TMEM142C: Key regulator or component of store-operated Ca(2+) channel and transcription factor NFAT nuclear import. Belongs to the Orai family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: integral to membrane

Molecular Function: protein binding