Western Blot (WB)
(Western blot analysis of ORAI2 in Jurkat cell lysate with ORAI2 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.)
Rabbit anti-Human, Mouse ORAI2 Polyclonal Antibody | anti-ORAI2 antibody
ORAI2 Antibody
Gene Names
ORAI2; CBCIP2; C7orf19; MEM142B; TMEM142B
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
ORAI2 Antibody is affinity chromatography purified via peptide column.
Synonyms
ORAI2; Polyclonal Antibody; ORAI2 Antibody; CBCIP2; C7orf19; MEM142B; TMEM142B; PP1729; Protein orai-2; CAP-binding protein complex-interacting protein 2; ORAI calcium release-activated calcium modulator 2; anti-ORAI2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
ORAI2 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
254
Applicable Applications for anti-ORAI2 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ORAI2 antibody can be used for detection of ORAI2 by Western blot at 1 - 4 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
ORAI2 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human ORAI2.
Buffer
ORAI2 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
ORAI2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of ORAI2 in Jurkat cell lysate with ORAI2 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.)
Western Blot (WB)
(Western blot analysis of ORAI2 in Jurkat cell lysate with ORAI2 antibody at (A) 1, (B) 2 and (C) 4 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of ORAI2 in Jurkat cells with ORAI2 antibody at 5 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of ORAI2 in Jurkat cells with ORAI2 antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ORAI2 in Jurkat cells with ORAI2 antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ORAI2 in Jurkat cells with ORAI2 antibody at 20 μg/mL.)
Related Product Information for anti-ORAI2 antibody
ORAI2 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI2 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. Like ORAI1, ORAI2 has been shown to function as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor, although at a lesser efficacy than ORAI1. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
28,570 Da
NCBI Official Full Name
Protein orai-2
NCBI Official Synonym Full Names
ORAI calcium release-activated calcium modulator 2
NCBI Official Symbol
ORAI2
NCBI Official Synonym Symbols
CBCIP2; C7orf19; MEM142B; TMEM142B
NCBI Protein Information
protein orai-2; H_NH0514P08.8; putative protein ORAI2-2; transmembrane protein 142B; CAP-binding protein complex interacting protein 2; CAP-binding protein complex-interacting protein 2
UniProt Protein Name
Protein orai-2
Protein Family
UniProt Gene Name
ORAI2
UniProt Synonym Gene Names
C7orf19; CBCIP2; TMEM142B
UniProt Entry Name
ORAI2_HUMAN
Uniprot Description
TMEM142B: Ca(2+) release-activated Ca(2+)-like (CRAC-like) channel subunit which mediates Ca(2+) influx and increase in Ca(2+)- selective current by synergy with the Ca(2+) sensor, STIM1. Belongs to the Orai family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 7q22.1
Cellular Component: growth cone; integral to membrane
Molecular Function: protein binding
Research Articles on ORAI2
Similar Products
Product Notes
The ORAI2 orai2 (Catalog #AAA150581) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ORAI2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ORAI2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). ORAI2 antibody can be used for detection of ORAI2 by Western blot at 1 - 4 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the ORAI2 orai2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ORAI2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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