Goat anti-Human MIA Polyclonal Antibody | anti-MIA antibody

Goat Polyclonal to Human MIA

Cat. Num. AAA248802

• Gene Names: MIA; CD-RAP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: MIA; Polyclonal Antibody; Goat Polyclonal to Human MIA; Anti-MIA Antibody (Internal) IHC-plus; CD-RAP; Melanoma inhibitory activity; Human MIA; anti-MIA antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human MIA. The immunizing peptide represents the N terminus of the mature protein. Reported variants represent identical protein: NP_006524.1, NP_001189482.1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 131

• Applicable Applications for anti-MIA antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:64000), IHC-P (5 ug/ml), WB (1 - 3 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 14kD band observed in Human Glioma lysates (calculated MW of 14.5kD according to NP_006524.1). Recommended concentration: 1-3 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide PKLADRKLCADQE from an internal region of human MIA (NP_006524.1). Percent identity by BLAST analysis: Hamster, Bovine, Rabbit, Pig (85%).
• Immunogen Type: Synthetic peptide
• Immunogen: MIA antibody was raised against synthetic peptide PKLADRKLCADQE from an internal region of human MIA (NP_006524.1). Percent identity by BLAST analysis: Hamster, Bovine, Rabbit, Pig (85%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-MIA / CD-RAP antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-MIA / CD-RAP antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(MIA antibody (1 ug/ml) staining of Human Glioma lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Product Categories/Family for anti-MIA antibody

Family: MIA

NCBI and Uniprot Product Information

• NCBI GI #: 5729925
• NCBI GeneID: 8190
• NCBI Accession #: NP_006524.1
• NCBI GenBank Nucleotide #: NM_006533.3
• UniProt Accession #: Q16674; Q6FHV3
• Molecular Weight: 6,361 Da
• NCBI Official Full Name: melanoma-derived growth regulatory protein
• NCBI Official Synonym Full Names: melanoma inhibitory activity
• NCBI Official Symbol: MIA
• NCBI Official Synonym Symbols: CD-RAP
• NCBI Protein Information: melanoma-derived growth regulatory protein
• UniProt Protein Name: Melanoma-derived growth regulatory protein
• Protein Family: Melanoma-derived growth regulatory protein
• UniProt Gene Name: MIA
• UniProt Entry Name: MIA_HUMAN

Uniprot Description

MIA: Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. Belongs to the MIA/OTOR family.

Protein type: Secreted, signal peptide; Cell adhesion; Secreted

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space

Molecular Function: growth factor activity

Biological Process: cell proliferation