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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NPAS3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

Rabbit anti-Human, Rat NPAS3 Polyclonal Antibody | anti-NPAS3 antibody

NPAS3 Rabbit pAb

Gene Names
NPAS3; MOP6; PASD6; bHLHe12
Reactivity
Human, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
NPAS3; Polyclonal Antibody; NPAS3 Rabbit pAb; MOP6; PASD6; bHLHe12; anti-NPAS3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-NPAS3 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant protein of human NPAS3.
Positive Samples
HepG2, MCF7, C6, SH-SY5Y, U-251MG, U-87MG
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using NPAS3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NPAS3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Related Product Information for anti-NPAS3 antibody
Background: This gene encodes a member of the basic helix-loop-helix and PAS domain-containing family of transcription factors. The encoded protein is localized to the nucleus and may regulate genes involved in neurogenesis. Chromosomal abnormalities that affect the coding potential of this gene are associated with schizophrenia and cognitive disability. Alternate splicing results in multiple transcript variants.
Product Categories/Family for anti-NPAS3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
28,563 Da
NCBI Official Full Name
Neuronal PAS domain-containing protein 3
NCBI Official Synonym Full Names
neuronal PAS domain protein 3
NCBI Official Symbol
NPAS3
NCBI Official Synonym Symbols
MOP6; PASD6; bHLHe12
NCBI Protein Information
neuronal PAS domain-containing protein 3; neuronal PAS3; member of PAS protein 6; PAS domain-containing protein 6; basic-helix-loop-helix-PAS protein MOP6; class E basic helix-loop-helix protein 12
UniProt Protein Name
Neuronal PAS domain-containing protein 3
UniProt Gene Name
NPAS3
UniProt Synonym Gene Names
BHLHE12; MOP6; PASD6; Neuronal PAS3; bHLHe12
UniProt Entry Name
NPAS3_HUMAN

NCBI Description

This gene encodes a member of the basic helix-loop-helix and PAS domain-containing family of transcription factors. The encoded protein is localized to the nucleus and may regulate genes involved in neurogenesis. Chromosomal abnormalities that affect the coding potential of this gene are associated with schizophrenia and mental retardation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

Uniprot Description

NPAS3: May play a broad role in neurogenesis. May control regulatory pathways relevant to schizophrenia and to psychotic illness. A chromosomal aberration involving NPAS3 is found in a family with schizophrenia. Translocation t(9;14)(q34;q13). 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 14q13.1

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein dimerization activity; signal transducer activity; DNA binding

Biological Process: startle response; transcription, DNA-dependent; maternal behavior; positive regulation of transcription, DNA-dependent; locomotory behavior; signal transduction

Research Articles on NPAS3

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Product Notes

The NPAS3 npas3 (Catalog #AAA9143736) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NPAS3 Rabbit pAb reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NPAS3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the NPAS3 npas3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NPAS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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