Typical Testing Data/Standard Curve (for reference only)
Chicken HLTF ELISA Kit | HLTF elisa kit
Chicken HLTF (Helicase Like Transcription Factor) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HLTF. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HLTF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HLTF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of HLTF. You can calculate the concentration of HLTF in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq, Jul 2008]
Uniprot Description
SMARCA3: an enzyme with apparent ATP-dependent DNA helicase activity. Belongs to the SNF2/RAD54 helicase family, RAD16 subfamily. Two alternatively spliced human isoforms have been described.
Protein type: DNA-binding; Ligase; EC 6.3.2.-; Nucleolus; EC 3.6.4.-; Ubiquitin conjugating system; EC 3.6.1.-; Helicase; EC 6.3.2.19; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 3q25.1-q26.1
Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; DNA binding; zinc ion binding; ATPase activity; helicase activity; ATP binding; ligase activity
Biological Process: regulation of transcription from RNA polymerase II promoter; protein polyubiquitination; transcription, DNA-dependent; regulation of transcription, DNA-dependent; postreplication repair; chromatin modification
Research Articles on HLTF
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Product Notes
The Chicken HLTF hltf (Catalog #AAA2501831) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2501831 ELISA Kit recognizes Chicken HLTF. It is sometimes possible for the material contained within the vial of "HLTF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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