Rabbit NPAS3 Polyclonal Antibody | anti-NPAS3 antibody

NPAS3 Antibody

Cat. Num. AAA150264

• Gene Names: NPAS3; MOP6; PASD6; bHLHe12
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: NPAS3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: NPAS3; Polyclonal Antibody; NPAS3 Antibody; MOP6; PASD6; bHLHe12; BHLHE12; Neuronal PAS domain-containing protein 3; Basic-helix-loop-helix-PAS protein MOP6; Neuronal PAS3; neuronal PAS domain protein 3; anti-NPAS3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: NPAS3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 933

• Applicable Applications for anti-NPAS3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: NPAS3 antibody can be used for detection of NPAS3 by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: NPAS3 antibody was raised against a 28 amino acid synthetic peptide from near the amino terminus of human NPAS3.
• Buffer: NPAS3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: NPAS3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of NPAS3 in SK-N-SH cell lysate with NPAS3 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of NPAS3 in human brain tissue with NPAS3 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of NPAS3 in human brain tissue with NPAS3 antibody at 20 μg/mL.)

Related Product Information for anti-NPAS3 antibody

NPAS3 Antibody: Neuronal PAS domain protein 3 (NPAS3) is a brain-enriched basic helix-loop-helix PAS domain transcription factor and is broadly expressed in the developing neuroepithelium and has recently found to be disrupted by genetic translocation in a family affected with schizophrenia. It was recently shown to be involved in the regulation of FGF signaling in the dentate gyrus by controlling the expression of the FGF receptor subtype 1 and in turn neurogenesis emanating from this region. NPAS3-null mice were growth-retarded and displayed brain defects that included reduced size of the anterior hippocampus, hypoplasia of the corpus callosum, and enlargement of the ventricles, as well as several behavioral abnormalities. Furthermore, these NPAS3-null mice also exhibited disruptions in several neurosignaling pathways involving glutamate, dopamine, and serotonin. These results demonstrate the essential role played by NPAS3 during structural and functional brain development. At least three isoforms of NPAS3 are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 38604888
• NCBI GeneID: 64067
• UniProt Accession #: Q8IXF0; Q86US6; Q86US7; Q8IXF2; Q9BY81; Q9H323; Q9Y4L8
• Molecular Weight: 28,563 Da
• NCBI Official Full Name: Neuronal PAS domain-containing protein 3
• NCBI Official Synonym Full Names: neuronal PAS domain protein 3
• NCBI Official Symbol: NPAS3
• NCBI Official Synonym Symbols: MOP6; PASD6; bHLHe12
• NCBI Protein Information: neuronal PAS domain-containing protein 3; neuronal PAS3; member of PAS protein 6; PAS domain-containing protein 6; basic-helix-loop-helix-PAS protein MOP6; class E basic helix-loop-helix protein 12
• UniProt Protein Name: Neuronal PAS domain-containing protein 3
• Protein Family: Neuronal PAS domain-containing protein
• UniProt Gene Name: NPAS3
• UniProt Synonym Gene Names: BHLHE12; MOP6; PASD6; Neuronal PAS3; bHLHe12
• UniProt Entry Name: NPAS3_HUMAN

NCBI Description

This gene encodes a member of the basic helix-loop-helix and PAS domain-containing family of transcription factors. The encoded protein is localized to the nucleus and may regulate genes involved in neurogenesis. Chromosomal abnormalities that affect the coding potential of this gene are associated with schizophrenia and mental retardation. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

Uniprot Description

NPAS3: May play a broad role in neurogenesis. May control regulatory pathways relevant to schizophrenia and to psychotic illness. A chromosomal aberration involving NPAS3 is found in a family with schizophrenia. Translocation t(9;14)(q34;q13). 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 14q13.1

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein dimerization activity; signal transducer activity; DNA binding

Biological Process: startle response; transcription, DNA-dependent; maternal behavior; positive regulation of transcription, DNA-dependent; locomotory behavior; signal transduction