Rabbit RCC1 Polyclonal Antibody | anti-RCC1 antibody

RCC1, phosphorylated (Ser11) (Rgulator of chromosome condensation 1, Cell cycle regulatory protein, CHC1, Chromosome condensation protein 1, RCC1-I, Regulator of chromosome condensation)

Cat. Num. AAA626053

• Gene Names: RCC1; CHC1; RCC1-I; SNHG3-RCC1
• Reactivity: Human, Monkey
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RCC1; Polyclonal Antibody; phosphorylated (Ser11) (Rgulator of chromosome condensation 1; Cell cycle regulatory protein; CHC1; Chromosome condensation protein 1; RCC1-I; Regulator of chromosome condensation); Anti -RCC1; anti-RCC1 antibody

• Host: Rabbit
• Reactivity: Human, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Phospho-RCC1when phosphorylated at Ser11. Species crossreactivity: monkey.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied in 10mM sodium HEPES pH 7.5, 150mM NaCl, 100ug/ml BSA and 50% glycerol.

• Applicable Applications for anti-RCC1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%; Tween-20 at 4 degree C with gentle shaking, overnight.
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser11 of human RCC1 protein(KLH-coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from HT-29 cells, untreated or synchronized in mitosis by thymidine block and released into nocodazole, using Phospho-RCC1 (Ser11) antibody (upper) or RCC1 antibody (lower).)

Related Product Information for anti-RCC1 antibody

The Ras family small GTPase Ran is involved in nuclear envelope formation, assembly of the mitotic spindle and nuclear transport (1,2). Like other small GTPases, Ran is active in its GTP-bound form and inactive in its GDP- bound form. Nuclear RanGTP concentration is maintained through nuclear localization of guanine nucleotide exchange factor (GEF) activity, which catalyzes the exchange of bound GDP for GTP. RCC1 (regulator of chromatin condensation 1) is the only known RanGEF (3). RCC1 is dynamically chromatin-bound throughout the cell cycle, and this localization is required for mitosis to proceed normally (4,5). Appropriate association of RCC1 with chromatin is regulated through amino-terminal phosphorylation (5,6) and methylation (7). RCC1 regulation of RanGTP levels in response to histone modifications regulates nuclear import during apoptosis (8). In mitosis RCC1 is phosphorylated at serine 11, possibly by cyclinB/cdc2 (9-11). This phosphorylation may play a role in RCC1 interaction with chromatin and its RanGEF activity (12).

Product Categories/Family for anti-RCC1 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 56206088
• NCBI GeneID: 1104
• UniProt Accession #: P18754; Q16269; Q6NT97; Q5T081
• Molecular Weight: 44,969 Da
• NCBI Official Full Name: regulator of chromosome condensation 1
• NCBI Official Synonym Full Names: regulator of chromosome condensation 1
• NCBI Official Symbol: RCC1
• NCBI Official Synonym Symbols: CHC1; RCC1-I; SNHG3-RCC1
• NCBI Protein Information: regulator of chromosome condensation; OTTHUMP00000003775; OTTHUMP00000003777; OTTHUMP00000211004; OTTHUMP00000211005; OTTHUMP00000211007; OTTHUMP00000211009; cell cycle regulatory protein; SNHG3-RCC1 readthrough transcript; guanine nucleotide-releasing protein
• UniProt Protein Name: Regulator of chromosome condensation
• Protein Family: RCC1 domain-containing protein
• UniProt Gene Name: RCC1
• UniProt Synonym Gene Names: CHC1
• UniProt Entry Name: RCC1_HUMAN

Uniprot Description

RCC1: a guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP for GTP. Localised generation of Ran-GTP by RCC1 on chromatin is critical for nucleocytoplasmic transport, mitotic spindle assembly and nuclear envelope formation. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo- cytoplasmic transport, mitosis and nuclear-envelope assembly. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; GEFs, Ras; GEFs

Chromosomal Location of Human Ortholog: 1p36.1

Cellular Component: nucleoplasm; nuclear membrane; condensed nuclear chromosome; nuclear chromatin; cytoplasm; nucleus

Molecular Function: protein binding; nucleosomal DNA binding; histone binding; Ran guanyl-nucleotide exchange factor activity; chromatin binding

Biological Process: mitosis; mitotic spindle organization and biogenesis; viral reproduction; cell division; regulation of mitosis; spindle assembly; chromosome segregation; G1/S transition of mitotic cell cycle