Western Blot (WB)
(Western Blot; Sample: Recombinant NEK2, Mouse.)
Rabbit anti-Mouse Never In Mitosis Gene A Related Kinase 2 (NEK2) Polyclonal Antibody | anti-NEK2 antibody
Polyclonal Antibody to Never In Mitosis Gene A Related Kinase 2 (NEK2)
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot; Sample: Recombinant NEK2, Mouse.)
Western Blot (WB)
(Western Blot; Sample: Recombinant NEK2, Mouse.)
Western Blot (WB)
(Western Blot; Sample: Rat Testis lysate;Primary Ab: 3ug/ml Rabbit Anti-Mouse NEK2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047).)
Western Blot (WB)
(Western Blot; Sample: Rat Testis lysate;Primary Ab: 3ug/ml Rabbit Anti-Mouse NEK2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047).)
Western Blot (WB)
(Western Blot; Sample: Human K562 cell lysate;Primary Ab: 3ug/ml Rabbit Anti-Mouse NEK2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047).)
Western Blot (WB)
(Western Blot; Sample: Human K562 cell lysate;Primary Ab: 3ug/ml Rabbit Anti-Mouse NEK2 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047).)
Western Blot (WB)
(Western Blot: Sample: Mouse Testis lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse NEK2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Western Blot (WB)
(Western Blot: Sample: Mouse Testis lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse NEK2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
Uniprot Description
NEK2: a protein kinase of the NEK family. Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation
Protein type: EC 2.7.11.1; Kinase, protein; NEK family; Nucleolus; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 1 H6|1 96.94 cM
Cellular Component: centrosome; chromosome; chromosome, centromeric region; condensed nuclear chromosome; cytoplasm; cytoskeleton; kinetochore; microtubule; midbody; nucleoplasm; nucleus; protein complex; spindle pole
Molecular Function: ATP binding; kinase activity; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein phosphatase binding; protein serine/threonine kinase activity; transferase activity
Biological Process: blastocyst development; cell cycle; cell division; centrosome separation; chromosome segregation; meiotic cell cycle; mitotic sister chromatid segregation; mitotic spindle assembly; negative regulation of DNA binding; phosphorylation; positive regulation of telomerase activity; positive regulation of telomere capping; positive regulation of telomere maintenance via telomerase; protein amino acid phosphorylation; protein autophosphorylation; regulation of attachment of spindle microtubules to kinetochore; regulation of mitotic centrosome separation
Research Articles on NEK2
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Product Notes
The NEK2 nek2 (Catalog #AAA2006320) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Never In Mitosis Gene A Related Kinase 2 (NEK2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Never In Mitosis Gene A Related Kinase 2 (NEK2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunoprecipitation (IP), Western Blot (WB). Western Blotting: 0.5-3ug/mL Immunohistochemistry: 5-30ug/mL Immunocytochemistry: 5-30ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the NEK2 nek2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Never In Mitosis Gene A Related Kinase 2 (NEK2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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