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Western Blot (WB) (Anti-WNT2 Picoband antibody, MBS178216, Western blottingAll lanes: Anti WNT2 (MBS178216) at 0.5ug/mlLane 1: COLO320 Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugPredicted bind size: 40KDObserved bind size: 40KD )

anti-Human Wnt2 Polyclonal Antibody | anti-WNT2 antibody

Anti-Wnt2 Antibody

Gene Names
WNT2; IRP; INT1L1
Reactivity
Human
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Wnt2; Polyclonal Antibody; Anti-Wnt2 Antibody; Protein Wnt-2; Int 1 related protein; Int-1-like protein 1; Int-1-related protein; INT1L 1; INT1L1; IRP; IRP protein; ONCOGENE INT1 LIKE 1; Secreted growth factor; Wingless type MMTV integration site family member 2; WNT 2; WNT2; WNT2_HUMAN; wingless-type MMTV integration site family member 2; anti-WNT2 antibody
Ordering
For Research Use Only!
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
360
Applicable Applications for anti-WNT2 antibody
Western Blot (WB)
Application Notes
Western Blot Concentration: 0.1-0.5ug/ml
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Wnt2 (56-81aa HRHPDVMRAISQGVAEWTAECQHQFR), different from the related mouse sequence by two amino acids.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-WNT2 Picoband antibody, MBS178216, Western blottingAll lanes: Anti WNT2 (MBS178216) at 0.5ug/mlLane 1: COLO320 Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugPredicted bind size: 40KDObserved bind size: 40KD )

Western Blot (WB) (Anti-WNT2 Picoband antibody, MBS178216, Western blottingAll lanes: Anti WNT2 (MBS178216) at 0.5ug/mlLane 1: COLO320 Whole Cell Lysate at 40ugLane 2: MCF-7 Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugPredicted bind size: 40KDObserved bind size: 40KD )
Related Product Information for anti-WNT2 antibody
Description: Rabbit IgG polyclonal antibody for Protein Wnt-2(WNT2) detection. Tested with WB in Human.

Background: WNT2, also known as Wingless-type MMTV integration site family, member 2, is a human gene. This gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Alternatively spliced transcript variants have been identified for this gene.
References
1. "Entrez Gene: WNT2 wingless-type MMTV integration site family, member 2" 2. Wainwright BJ, Scambler PJ, Stanier P, Watson EK, Bell G, Wicking C, Estivill X, Courtney M, Boue A, Pedersen PS (June 1988). "Isolation of a human gene with protein sequence similarity to human and murine int-1 and the Drosophila segment polarity mutant wingless". EMBO J. 7(6): 1743-8.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,418 Da
NCBI Official Full Name
protein Wnt-2
NCBI Official Synonym Full Names
Wnt family member 2
NCBI Official Symbol
WNT2
NCBI Official Synonym Symbols
IRP; INT1L1
NCBI Protein Information
protein Wnt-2
UniProt Protein Name
Protein Wnt-2
UniProt Gene Name
WNT2
UniProt Synonym Gene Names
INT1L1; IRP; IRP
UniProt Entry Name
WNT2_HUMAN

NCBI Description

This gene is a member of the WNT gene family. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT2: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted; Cell development/differentiation; Cell adhesion

Chromosomal Location of Human Ortholog: 7q31.2

Cellular Component: cytoplasm; extracellular matrix; extracellular region; extracellular space; plasma membrane; proteinaceous extracellular matrix

Molecular Function: cytokine activity; frizzled binding; protein binding; receptor agonist activity

Biological Process: atrial cardiac muscle morphogenesis; cell fate commitment; cell proliferation in midbrain; cell-cell signaling; lens development in camera-type eye; lung development; neurogenesis; neuron differentiation; positive regulation of cardiac muscle cell proliferation; positive regulation of cell proliferation; positive regulation of endothelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of neurogenesis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin

Research Articles on WNT2

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Product Notes

The WNT2 wnt2 (Catalog #AAA178216) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Wnt2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Wnt2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the WNT2 wnt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wnt2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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