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Western Blot (WB) (Western Blot: Sample: Human 293T cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse IL2Rb Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)

Rabbit anti-Mouse Interleukin 2 Receptor Beta (IL2Rb) Antibody | anti-IL2Rb antibody

Polyclonal Antibody to Interleukin 2 Receptor Beta (IL2Rb)

Gene Names
Il2rb; p70; CD122; IL15Rbeta; Il-2Rbeta; IL-15Rbeta; Il-2/15Rbeta
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Synonyms
Interleukin 2 Receptor Beta (IL2Rb); Polyclonal Antibody to Interleukin 2 Receptor Beta (IL2Rb); anti-IL2Rb antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.37mg/ml (varies by lot)
Applicable Applications for anti-IL2Rb antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-3ug/mL
Immunohistochemistry: 5-30ug/mL
Immunocytochemistry: 5-30ug/mL
Optimal working dilutions must be determined by end user.
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Human, Mouse
Immunogen
Recombinant IL2Rb (Ala27~Glu240) expressed in E.coli (MBS2010767).
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 24 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Human 293T cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse IL2Rb Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)

Western Blot (WB) (Western Blot: Sample: Human 293T cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse IL2Rb Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)

Western Blot (WB)

(Western Blot: Sample: Human Hela cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse IL2Rb Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(MBS2086047))

Western Blot (WB) (Western Blot: Sample: Human Hela cell lysate; Primary Ab: 3ug/ml Rabbit Anti-Mouse IL2Rb Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;(MBS2086047))

Western Blot (WB)

(Figure. Western Blot; Sample: Recombinant IL2Rb, Mouse.)

Western Blot (WB) (Figure. Western Blot; Sample: Recombinant IL2Rb, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Mouse Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P. Samples: Mouse Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interleukin-2 receptor subunit beta
NCBI Official Synonym Full Names
interleukin 2 receptor, beta chain
NCBI Official Symbol
Il2rb
NCBI Official Synonym Symbols
p70; CD122; IL15Rbeta; Il-2Rbeta; IL-15Rbeta; Il-2/15Rbeta
NCBI Protein Information
interleukin-2 receptor subunit beta
UniProt Protein Name
Interleukin-2 receptor subunit beta
Protein Family
UniProt Gene Name
Il2rb
UniProt Synonym Gene Names
IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB

NCBI Description

The interleukin 2 receptor is composed of alpha and beta subunits. The beta subunit encoded by this gene is very homologous to the human beta subunit and also shows structural similarity to other cytokine receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

IL2RB: Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. Interacts with SHB upon interleukin stimulation. Interacts with HTLV-1 accessory protein p12I. Belongs to the type I cytokine receptor family. Type 4 subfamily.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 15 E1|15 37.52 cM

Cellular Component: cell surface; external side of plasma membrane; integral component of membrane; membrane

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-2 binding; interleukin-2 receptor activity

Biological Process: cytokine-mediated signaling pathway; natural killer cell activation; negative regulation of apoptosis

Research Articles on IL2Rb

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Product Notes

The IL2Rb il2rb (Catalog #AAA2002997) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Interleukin 2 Receptor Beta (IL2Rb) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 2 Receptor Beta (IL2Rb) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western blotting: 0.5-3ug/mL Immunohistochemistry: 5-30ug/mL Immunocytochemistry: 5-30ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the IL2Rb il2rb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 2 Receptor Beta (IL2Rb), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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