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Western Blot (WB) (Western Blot detection against Immunogen (33.92kD).)

Mouse anti-Human ZIC4 Monoclonal Antibody | anti-ZIC4 antibody

ZIC4 (Zinc Finger Protein of the Cerebellum 4, Zinc Finger Protein ZIC 4)

Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
ZIC4; Monoclonal Antibody; ZIC4 (Zinc Finger Protein of the Cerebellum 4; Zinc Finger Protein ZIC 4); Anti -ZIC4 (Zinc Finger Protein of the Cerebellum 4; anti-ZIC4 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1A9
Specificity
Recognizes human ZIC4.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
SDRKKHSHVHTSDKPYTCKVRGCDKCYTHPSSLRKHMKVHGRSPPPSSGYDSATPSALVSPSSDCGHKSQV
Applicable Applications for anti-ZIC4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
Immunogen
Partial recombinant corresponding to aa249-320 from human ZIC4 (NP_115529) with GST tag. MW of the GST tag alone is 26kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (33.92kD).)

Western Blot (WB) (Western Blot detection against Immunogen (33.92kD).)

Western Blot (WB)

(ZIC4 monoclonal antibody, Western Blot analysis of ZIC4 expression in Hela NE.)

Western Blot (WB) (ZIC4 monoclonal antibody, Western Blot analysis of ZIC4 expression in Hela NE.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to ZIC4 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)

Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to ZIC4 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ZIC4 on HeLa cell. [antibody concentration 10ug/ml].)

Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to ZIC4 on HeLa cell. [antibody concentration 10ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged ZIC4 is ~0.03ng/ml as a capture antibody)

Testing Data (Detection limit for recombinant GST tagged ZIC4 is ~0.03ng/ml as a capture antibody)
Product Categories/Family for anti-ZIC4 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,567 Da
NCBI Official Full Name
zinc finger protein ZIC 4 isoform 4
NCBI Official Synonym Full Names
Zic family member 4
NCBI Official Symbol
ZIC4
NCBI Protein Information
zinc finger protein ZIC 4; zinc family member 4 protein HZIC4; zinc finger protein of the cerebellum 4
UniProt Protein Name
Zinc finger protein ZIC 4
Protein Family
UniProt Gene Name
ZIC4
UniProt Entry Name
ZIC4_HUMAN

NCBI Description

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development, and have been associated with X-linked visceral heterotaxy and holoprosencephaly type 5. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 1, a related family member on chromosome 3. Heterozygous deletion of these linked genes is involved in Dandy-Walker malformation, which is a congenital cerebellar malformation. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Dec 2009]

Uniprot Description

Function: Binds to DNA

By similarity.

Subcellular location: Nucleus

By similarity.

Sequence similarities: Belongs to the GLI C2H2-type zinc-finger protein family.Contains 5 C2H2-type zinc fingers.

Caution: It is uncertain whether Met-1 is the initiator.

Sequence caution: The sequence AAH29507.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence starting in position 229.The sequence AAK06845.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Research Articles on ZIC4

Similar Products

Product Notes

The ZIC4 zic4 (Catalog #AAA647508) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ZIC4 (Zinc Finger Protein of the Cerebellum 4, Zinc Finger Protein ZIC 4) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZIC4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Dilution: Immunofluorescence: 10ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml. Researchers should empirically determine the suitability of the ZIC4 zic4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SDRKKHSHVH TSDKPYTCKV RGCDKCYTHP SSLRKHMKVH GRSPPPSSGY DSATPSALVS PSSDCGHKSQ V. It is sometimes possible for the material contained within the vial of "ZIC4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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