Testing Data
Chicken anti-Rat Neurofilament NF-L ck Polyclonal Antibody | anti-NEFL antibody
Anti-Neurofilament L (NF-L)
Reactivity
Rat
Applications
Western Blot, Immunofluorescence
Purity
Total IgY fraction
Synonyms
Neurofilament NF-L ck; Polyclonal Antibody; Anti-Neurofilament L (NF-L); anti-NEFL antibody
Host
Chicken
Reactivity
Rat
Clonality
Polyclonal
Specificity
Specific for the ~68k Neurofilament L protein in Western blots and works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections.
Purity/Purification
Total IgY fraction
Form/Format
Liquid containing 10mM sodium azide.
Sequence Length
555
Applicable Applications for anti-NEFL antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Quality Control: Western blots performed on each lot.
WB: 1:10,000
IF: 1:5,000
WB: 1:10,000
IF: 1:5,000
Antigen
Preparation of bovine spinal cord intermediate filaments
Immunogen Information
Preparation of bovine spinal cord NF-L
Immunogen Species
Bovine
Reactivity Assumed Based on 100% Sequence Homology
Chicken, human, mouse
Species Reactivity Note
The antibody has been directly tested for reactivity in bovine, chicken, human, mouse and rat tissues.
Biological Significance
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (2).
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
Testing Data
Testing Data
Related Product Information for anti-NEFL antibody
Chicken polyclonal antibody
References
• Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.
• Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. (2000) A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet. 67:37-46.
• Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. (2000) A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet. 67:37-46.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68
NCBI Official Full Name
neurofilament light polypeptide
NCBI Official Synonym Full Names
neurofilament, light polypeptide
NCBI Official Symbol
NEFL
NCBI Protein Information
neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; neurofilament triplet L protein; micro glutamic acid-rich protein; neurofilament, light polypeptide 68kDa
UniProt Protein Name
Neurofilament light polypeptide
Protein Family
UniProt Gene Name
NEFL
UniProt Synonym Gene Names
NF-L
UniProt Entry Name
NFL_BOVIN
Uniprot Description
Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Subunit structure: Interacts with ARHGEF28
By similarity. Interacts with TRIM2
By similarity.
Domain: The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.
Post-translational modification: O-glycosylated
By similarity.Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization
By similarity. Ref.4Ubiquitinated in the presence of TRIM2 and UBE2D1
By similarity.
Miscellaneous: NF-L is the most abundant of the three neurofilament proteins and, like the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.
Sequence similarities: Belongs to the intermediate filament family.
Mass spectrometry: Molecular mass is 62600 Da from positions 2 - 555. Determined by MALDI. Ref.4
Similar Products
Product Notes
The NEFL nefl (Catalog #AAA502142) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Anti-Neurofilament L (NF-L) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neurofilament NF-L ck can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Quality Control: Western blots performed on each lot. WB: 1:10,000 IF: 1:5,000. Researchers should empirically determine the suitability of the NEFL nefl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurofilament NF-L ck, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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