Testing Data
Chicken anti-Rat Peripherin ck Polyclonal Antibody | anti-PRPH antibody
Anti-Peripherin
Gene Names
Prph; Perf; Prph1
Reactivity
Rat
Applications
Western Blot, Immunofluorescence
Purity
Total IgY fraction
Synonyms
Peripherin ck; Polyclonal Antibody; Anti-Peripherin; anti-PRPH antibody
Host
Chicken
Reactivity
Rat
Clonality
Polyclonal
Specificity
Specific for the ~57kDa peripherin protein.
Purity/Purification
Total IgY fraction
Form/Format
Contains 10 mM sodium azide.
Sequence Length
468
Applicable Applications for anti-PRPH antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Quality Control: Western blots performed on each lot
WB:1:1,000
IF: 1:500
WB:1:1,000
IF: 1:500
Antigen
Recombinant rat peripherin expressed in and purified from E. coli.
Immunogen Information
Recombinant rat peripherin expressed in and purified from E. coli
Immunogen Species
Rat
Reactivity Assumed Based on 100% Sequence Homology
Many mammalian
Species Reactivity Note
The antibody has been directly tested for reactivity in many mammalian tissues.
Biological Significance
Peripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems, which gives the protein its name. Subsequently, peripherin was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which also includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. In contrast to the neurofilaments, peripherin is strongly up-regulated after nerve injury (1). Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors (2). Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes (3). Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.
Testing Data
Testing Data
Related Product Information for anti-PRPH antibody
Chicken polyclonal antibody
References
• Terao E, Janssens S, van den Bosch de Aquilar P, Portier M, Klosen P (2000) In vivo expression of the intermediate filament peripherin in rat motorneurons: modulation by inhibitory and stimulatory signals. Neuroscience 101(3):679-88.
• Roberson J, Doroudchi MM, Nguyen MD, Durham, HD, Shaw G, Julien JP, Mushynski WE. (2003) A neurotoxic peripherin splice variant in a mouse model of ALS. J. Cell Biol. 160(6):939-49.
• Boitard C, Villa MC, Becourt C, Gia HP, Huc C, Sempe P, Portier MM, Bach JF (1992) Peripherin: an islet antigen that is cross-reactive with nonobese diabetic mouse class II gene products. Proc Natl Acad Sci USA 89(1):172-6.
• Roberson J, Doroudchi MM, Nguyen MD, Durham, HD, Shaw G, Julien JP, Mushynski WE. (2003) A neurotoxic peripherin splice variant in a mouse model of ALS. J. Cell Biol. 160(6):939-49.
• Boitard C, Villa MC, Becourt C, Gia HP, Huc C, Sempe P, Portier MM, Bach JF (1992) Peripherin: an islet antigen that is cross-reactive with nonobese diabetic mouse class II gene products. Proc Natl Acad Sci USA 89(1):172-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
57
NCBI Official Full Name
Peripherin
NCBI Official Synonym Full Names
peripherin
NCBI Official Symbol
Prph
NCBI Official Synonym Symbols
Perf; Prph1
NCBI Protein Information
peripherin; peripherin 1
UniProt Protein Name
Peripherin
Protein Family
UniProt Gene Name
Prph
UniProt Synonym Gene Names
Prph1
UniProt Entry Name
PERI_RAT
NCBI Description
contributes to the formation of intermediate filament networks [RGD, Feb 2006]
Uniprot Description
Function: Class-III neuronal intermediate filament protein.
Sequence similarities: Belongs to the intermediate filament family.
Research Articles on PRPH
Similar Products
Product Notes
The PRPH prph (Catalog #AAA502144) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Anti-Peripherin reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Peripherin ck can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Quality Control: Western blots performed on each lot WB:1:1,000 IF: 1:500. Researchers should empirically determine the suitability of the PRPH prph for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peripherin ck, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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