Mouse Neurofilament NF-H Monoclonal Antibody

Neurofilament NF-H Antibody

Cat. Num. AAA415191

• Applications: Immunofluorescence, Western Blot
• Purity: Affinity purified
• Synonyms: Neurofilament NF-H; Monoclonal Antibody; Neurofilament NF-H Antibody; anti-Neurofilament NF-H antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: The antibody recognizes NF-H strongly in all mammals tested to date and also in chicken.
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)

• Applicable Applications for anti-Neurofilament NF-H antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: 1:500 in immunofluorescence experiments. In western blotting using chemiluminescence it can be used at dilutions of 1:5000.
• Immunogen: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in the neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (also known as Lou Gehrig's disease) and Alzheimer's disease. MCA-AH1 is one of numerous antibodies which reacts preferentially with the axonal phosphorylated forms of NF-H. Interestingly these phosphorylated forms of NF-H are normally restricted to axons, while less phosphorylated forms are found in dendrites. However in numerous damage and disease states, phosphorylated NF-H can be detected with MCA-AH1 in dendritic and perikaryal neurofilaments.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Testing Data #2

References

Harris, J., Ayyub, C. and Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62 1991.