Rabbit anti-Human MAP4K1 Polyclonal Antibody | anti-MAP4K1 antibody

MAP4K1, CT (Mitogen-activated Protein Kinase Kinase Kinase Kinase 1, MAPK/ERK Kinase Kinase Kinase 1, MEK Kinase Kinase 1, MEKKK 1, Hematopoietic Progenitor Kinase, HPK1) (MaxLight 550)

Cat. Num. AAA6488253

• Gene Names: MAP4K1; HPK1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: MAP4K1; Polyclonal Antibody; CT (Mitogen-activated Protein Kinase Kinase Kinase Kinase 1; MAPK/ERK Kinase Kinase Kinase 1; MEK Kinase Kinase 1; MEKKK 1; Hematopoietic Progenitor Kinase; HPK1) (MaxLight 550); EC=2.7.11.1; Hematopoietic Progenitor Kinase 1; anti-MAP4K1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAP4K1.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
• Sequence Length: 833

• Applicable Applications for anti-MAP4K1 antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 321-350 in the C-terminal region of human MAP4K1, Swiss/UniProt Accession: Q92918.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of hHPK1-C335 in Ramos cell line lysates (35ug/lane) usingMBS628049. HPK1 (arrow) was detected usingMBS628049.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human cancer tissue using MBS628049 which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS628049 for immunohistochemistry. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-MAP4K1 antibody

Antibodies; Protein Kinases

References

1. Sawasdikosol, S., et al., Blood 101(9):3687-3689 (2003). 2. Hu, M.C., et al., Genes Dev. 10(18):2251-2264 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 6005810
• NCBI GeneID: 11184
• NCBI Accession #: NP_009112
• NCBI GenBank Nucleotide #: NP_009112.1
• UniProt Accession #: Q92918
• Molecular Weight: 91.296
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 1 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 1
• NCBI Official Symbol: MAP4K1
• NCBI Official Synonym Symbols: HPK1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 1
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: MAP4K1
• UniProt Synonym Gene Names: HPK1; MEK kinase kinase 1; MEKKK 1
• UniProt Entry Name: M4K1_HUMAN

Uniprot Description

HPK1: a protein kinase of the STE20 family. Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. May play a role in hematopoietic lineage decisions and growth regulation. Positively regulated by PGE2 and a negative regulator of PGE2-induced FOS gene transcription.

Protein type: EC 2.7.11.1; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; STE group; STE20 family; KHS subfamily

Chromosomal Location of Human Ortholog: 19q13.1-q13.4

Cellular Component: membrane; cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase kinase activity; ATP binding; protein kinase activity

Biological Process: cell proliferation; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; activation of MAPKKK activity; response to stress; protein amino acid phosphorylation; activation of JNK activity