Western Blot (WB)
(Western Blot analysis of NET1 expression in transfected 293T cell line by NET1 polyclonal antibody. Lane 1: NET1 transfected lysate (61.9kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human NET1 Polyclonal Antibody | anti-NET1 antibody
NET1 (Neuroepithelial Cell-transforming Gene 1 Protein, Proto-oncogene p65 Net1, Rho Guanine Nucleotide Exchange Factor 8, ARHGEF8) (AP)
Gene Names
NET1; NET1A; ARHGEF8
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NET1; Polyclonal Antibody; NET1 (Neuroepithelial Cell-transforming Gene 1 Protein; Proto-oncogene p65 Net1; Rho Guanine Nucleotide Exchange Factor 8; ARHGEF8) (AP); anti-NET1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human NET1.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-NET1 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full-length human NET1, aa1-542 (NP_005854.2).
Immunogen Sequence
MVAHDETGGLLPIKRTIRVLDVNNQSFREQEEPSNKRVRPLARVTSLANLISPVRNGAVRRFGQTIQSFTLRGDHRSPASAQKFSSRSTVPTPAKRRSSALWSEMLDITMKESLTTREIRRQEAIYEMSRGEQDLIEDLKLARKAYHDPMLKLSIMSEEELTHIFGDLDSYIPLHEDLLTRIGEATKPDGTVEQIGHILVSWLPRLNAYRGYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILKHTPKEHPDVQLLEDAILIIQGVLSDINLKKGESECQYYIDKLEYLDEKQRDPRIEASKVLLCHGELRSKSGHKLYIFLFQDILVLTRPVTRNERHSYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFSNSEKAKNIFRIRFHDPSPAQSHTLQANDVFHKQQWFNCIRAAIAPFQSAGSPPELQGLPELHEECEGNHPSARKLTAQRRASTVSSVTQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of NET1 expression in transfected 293T cell line by NET1 polyclonal antibody. Lane 1: NET1 transfected lysate (61.9kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of NET1 expression in transfected 293T cell line by NET1 polyclonal antibody. Lane 1: NET1 transfected lysate (61.9kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-NET1 antibody
Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
Product Categories/Family for anti-NET1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,885 Da
NCBI Official Full Name
Homo sapiens neuroepithelial cell transforming 1 (NET1), transcript variant 2, mRNA
NCBI Official Synonym Full Names
neuroepithelial cell transforming 1
NCBI Official Symbol
NET1
NCBI Official Synonym Symbols
NET1A; ARHGEF8
NCBI Protein Information
neuroepithelial cell-transforming gene 1 protein; Rho guanine nucleotide exchange factor (GEF) 8; guanine nucleotide regulatory protein (oncogene); neuroepithelioma transforming gene 1; p65 Net1 proto-oncogene protein; small GTP-binding protein regulator
Protein Family
NCBI Description
This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]
Research Articles on NET1
Similar Products
Product Notes
The NET1 (Catalog #AAA6386875) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NET1 (Neuroepithelial Cell-transforming Gene 1 Protein, Proto-oncogene p65 Net1, Rho Guanine Nucleotide Exchange Factor 8, ARHGEF8) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NET1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NET1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NET1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
