Rabbit NDUFV2 Polyclonal Antibody | anti-NDUFV2 antibody

Anti-NDUFV2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8109367

• Reactivity: Human, Cynomolgus
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: NDUFV2; Polyclonal Antibody; Anti-NDUFV2 Antibody; Rabbit Polyclonal; NDUFV2 Antibody; Rabbit PAb; Antigen Affinity Purified; NADH:ubiquinone oxidoreductase core subunit V2; Anti-CI-24k Antibody; anti-NDUFV2 antibody

• Host: Rabbit
• Reactivity: Human, Cynomolgus
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NDUFV2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-NDUFV2 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human NDUFV2 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human NDUFV2 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NDUFV2 rabbit polyclonal antibody at 1:500 dilution Lane A: A549 Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lane C: HeLa Whole Cell Lysate Lane D: K-562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:27 kDa Observed band size:26 kDa (We are unsure as to the identity of these extra bands.))

Immunohistochemistry (IHC)

(Immunochemical staining of human NDUFV2 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human NDUFV2 in cynomolgus heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(NDUFV2 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-NDUFV2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-NDUFV2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 25 kDa Observed band size :25 kDa)

Related Product Information for anti-NDUFV2 antibody

NDUFV2, located on 18p11.31-p11.2, encodes an important subunit of mitochondrial NADH (complex I). NADH dehydrogenase (ubiquinone) flavoprotein 2 (NDUFV2), containing one iron-sulfur cluster ([2Fe-2S] binuclear cluster N1a), is one of the core nuclear-encoded subunits existing in human mitochondrial complex I. Defects in this subunit have been associated with Parkinson's disease, Alzheimer's disease, Bipolar disorder, and Schizophrenia. The mitochondrial targeting sequence of NDUFV2 is located at the N-terminus of the precursor protein. Maintaining a net positive charge and an amphiphilic structure with the overall balance and distribution of basic and hydrophobic amino acids in the N-terminus of NDUFV2 is important for mitochondrial targeting. The results of the human disease cell model established that the impairment of mitochondrial localization of NDUFV2 as a mechanistic basis for early-onset hypertrophic cardiomyopathy and encephalopathy.

References

Zhang J, et al. (2009) Association study on the mitochondrial gene ndufv2 and bipolar disorder in the chinese han population. J Neural Transm (Vienna) 116 (3): 357-361.Nishioka K, et al. (2010) Genetic variation of the mitochondrial complex i subunit ndufv2 and parkinson's disease. Parkinsonism Relat Disord 16 (10): 686-687.Liu HY, et al. (2011) Mitochondrial targeting of human nadh dehydrogenase (ubiquinone) flavoprotein 2 (ndufv2) and its association with early-onset hypertrophic cardiomyopathy and encephalopathy. J Biomed Sci 18 (1): 29.