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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ATF4 antibody.)

Rabbit ATF4 Polyclonal Antibody | anti-ATF4 antibody

ATF4 Antibody

Gene Names
ATF4; CREB2; TXREB; CREB-2; TAXREB67
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
ATF4; Polyclonal Antibody; ATF4 Antibody; CREB-2; CREB2; TAXREB67; TXREB; anti-ATF4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen affinity purification
Concentration
1mg/mL (varies by lot)
Sequence Length
351
Applicable Applications for anti-ATF4 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Immunogen
Recombinant protein of human ATF4
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-7583 / sc-200 / sc-22800
Preparation and Storage
Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ATF4 antibody.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ATF4 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach cancer using ATF4 antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach cancer using ATF4 antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse liver using ATF4 antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse liver using ATF4 antibody at dilution of 1:200 (200x lens).)
Related Product Information for anti-ATF4 antibody
ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. This antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
468
UniProt Accession #
Molecular Weight
38,590 Da
NCBI Official Full Name
ATF4
NCBI Official Synonym Full Names
activating transcription factor 4
NCBI Official Symbol
ATF4
NCBI Official Synonym Symbols
CREB2; TXREB; CREB-2; TAXREB67
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-4; DNA-binding protein TAXREB67; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; cyclic AMP-responsive element-binding protein 2; tax-responsive enhancer element B67; tax-responsive enhancer element-binding protein 67
UniProt Protein Name
ATF4 protein
UniProt Gene Name
ATF4
UniProt Entry Name
Q96AQ3_HUMAN

NCBI Description

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]

Research Articles on ATF4

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Product Notes

The ATF4 atf4 (Catalog #AAA2528151) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATF4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATF4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the ATF4 atf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATF4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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