Western Blot (WB)
(Anti-NDRG3-1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa Observed band size:45 kDa)
Rabbit anti-Human NDRG3 Polyclonal Antibody | anti-NDRG3 antibody
Anti-NDRG3 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
NDRG3; Polyclonal Antibody; Anti-NDRG3 Antibody; Rabbit Polyclonal; NDRG3 Antibody; Rabbit PAb; Antigen Affinity Purified; NDRG family member 3; Anti-RP3-460J8.1 Antibody; anti-NDRG3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human NDRG3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-NDRG3 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
E Coli-derived Human NDRG3 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human NDRG3 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-NDRG3-1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa Observed band size:45 kDa)
Western Blot (WB)
(Anti-NDRG3-1 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:41 kDa Observed band size:45 kDa)
Related Product Information for anti-NDRG3 antibody
N-Myc downstream-regulated gene 3 (NDRG3) is one of the important members of the NDRG family which crucially take part in cell proliferation, differentiation, and other biological processes. NDRG3 plays important role in human cancer, and its expression may be considered as an oncogenic biomarker and a novel predictor for HCC prognosis. For example, NDRG3 facilitates colorectal cancer (CRC) migration and invasion by activating Src phosphorylation, suggesting the role of NDRG3 as a candidate oncogene; NDRG3 downregulation might be involved in the tumorigenesis and development of invasive breast cancer (BC) in an advanced phase of the disease. NDRG3 belongs to the N-Myc down-regulated gene (NDRG) family that contains 4 paralogs: NDRG1, -2, -3, and -4.
References
Wang W, et al. (2009) Ndrg3 is an androgen regulated and prostate enriched gene that promotes in vitro and in vivo prostate cancer cell growth. Int J Cancer 124 (3): 521-530.Luo X, et al. (2018) High expression of ndrg3 associates with unfavorable overall survival in non-small cell lung cancer. Cancer Biomark 21 (2): 461-469.Jing JS, et al. (2018) Ndrg3 overexpression is associated with a poor prognosis in patients with hepatocellular carcinoma. Biosci Rep 38 (6): BSR20180907.
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Product Notes
The NDRG3 (Catalog #AAA8108594) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NDRG3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NDRG3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the NDRG3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDRG3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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