Western Blot (WB)
(Anti-TBK1 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:84 kDa Observed band size:87 kDa)
Rabbit anti-Human TBK1 Polyclonal Antibody | anti-TBK1 antibody
Anti-TBK1 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
TBK1; Polyclonal Antibody; Anti-TBK1 Antibody; Rabbit Polyclonal; TBK1 Antibody; Rabbit PAb; Antigen Affinity Purified; TANK-binding kinase 1; Anti-NAK Antibody; Anti-T2K Antibody; anti-TBK1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TBK1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-TBK1 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
E Coli-derived Human TBK1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human TBK1 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-TBK1 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:84 kDa Observed band size:87 kDa)
Western Blot (WB)
(Anti-TBK1 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:84 kDa Observed band size:87 kDa)
Related Product Information for anti-TBK1 antibody
TANK-binding kinase 1 (TBK1) is an important serine/threonine-protein kinase that mediates phosphorylation and nuclear translocation of IRF3, which contributes to the induction of type I interferons (IFNs) in the innate antiviral response. TBK1 is a noncanonical IkappaB kinase family member that regulates the innate immune response. Misregulation of TBK1 activity can promote inflammatory disorders and oncogenesis; therefore, TBK1 inhibitors are considered a promising therapy for inflammation and cancer. In mammals, TBK1 spliced isoform negatively regulates the virus-triggered IFN-beta signaling pathway by disrupting the interaction between retinoic acid-inducible gene I (RIG-I) and mitochondria antiviral-signaling protein (MAVS). TBK1 plays a crucial role as the hub for many innate immune signaling pathways. Due to its key function in maintaining homeostasis of the immune system, cell survival, and proliferation, TBK1 activity is tightly regulated.
Product Categories/Family for anti-TBK1 antibody
References
Yu T, et al. (2015) Tbk1 inhibitors: A review of patent literature (2011-2014). Expert Opin Ther Pat 25 (12): 1385-1396.Li S, et al. (2019) Zebrafish mvp recruits and degrades tbk1 to suppress ifn production. J Immunol 202 (2): 559-566.Hu YW, et al. (2018) Tank-binding kinase 1 (tbk1) isoforms negatively regulate type i interferon induction by inhibiting tbk1-irf3 interaction and irf3 phosphorylation. Front Immunol 9 84.Hasan M, et al. (2016) Therapeutic potential of targeting tbk1 in autoimmune diseases and interferonopathies. Pharmacol Res 111 336-342.
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Product Notes
The TBK1 (Catalog #AAA8109579) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TBK1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TBK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the TBK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TBK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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