Rabbit anti-Human DEK Polyclonal Antibody | anti-DEK antibody
Anti-DEK Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
DEK; Polyclonal Antibody; Anti-DEK Antibody; Rabbit Polyclonal; DEK Antibody; Rabbit PAb; Antigen Affinity Purified; DEK proto-oncogene; Anti-D6S231E Antibody; anti-DEK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human DEK
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-DEK antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
E Coli-derived Human DEK fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human DEK fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-DEK rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:50 kDa (We are unsure as to the identity of these extra bands.))
Related Product Information for anti-DEK antibody
The DNA binding protein and chromatin structural regulator DEK regulate many cellular processes. These include proliferation, differentiation, apoptosis, senescence, DNA repairing and the maintenance of stem cell phenotype. DEK is increasingly recognized as a crucial player in many steps of cancer initiation and progression, and is precisely regulated by abundant promoting and inhibiting factors directly or indirectly. DEK may serve as an architectural modulating protein to regulate the expression and function of multiple human genes in cancer cells. DEK is a highly conserved chromatin-bound protein whose upregulation across cancer types correlates with genotoxic therapy resistance. Loss of DEK induces genome instability and sensitizes cells to DNA double strand breaks (DSBs), suggesting defects in DNA repair. The DEK oncogene is overexpressed in many human malignancies including at early tumor stages. The high levels of DEK significantly reprogrammed cellular metabolism and altered the abundances of amino acids, TCA cycle intermediates and the glycolytic end products lactate, alanine and NAD+. Decreased DEK expression in plasma cells suggests a potential role of this gene in plasma cell development and lack of detectable DEK protein by IHC could be used as a biomarker for normal and malignant plasma cells.
